Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Life Science Research Center, Yulin University, Yulin, China.
Int J Biol Sci. 2019 May 20;15(7):1368-1382. doi: 10.7150/ijbs.33233. eCollection 2019.
Recent studies have demonstrated that dermal papilla cell-derived exosomes (DPC-Exos) promote the anagen stage of hair follicle (HF) growth and delay the catagen stage. However, the roles of DPC-Exos in regulating hair follicle stem cell (HFSC) quiescence and activation remain unknown. Here, we found that HFSC differentiation was induced by co-culture with DPCs, and that DPC-Exos attached to the surface of HFSCs. Using micro RNA (miRNA) high-throughput sequencing, we identified 111 miRNAs that were significantly differentially expressed between DPC-Exos and DPCs, and the predicted target genes of the top 34 differentially expressed miRNAs indicated that DPC-Exos regulate HFSCs proliferation and differentiation via genes involved in cellular signal transduction, fatty acid expression regulation, and cellular communication. The overexpression of miR-22-5p indicated that it negatively regulates HFSC proliferation and was revealed as the direct target gene of miR-22-5p. We therefore propose the miR-22-5p- axis as a novel pathway regulating HFSC proliferation.
最近的研究表明，真皮乳头细胞衍生的外泌体（DPC-Exos）促进毛囊（HF）生长的生长期并延缓退行期。然而，DPC-Exos 在调节毛囊干细胞（HFSC）静止和激活中的作用尚不清楚。在这里，我们发现 HFSC 分化是由与 DPC 共培养诱导的，并且 DPC-Exos 附着在 HFSC 的表面。通过 micro RNA（miRNA）高通量测序，我们鉴定出 111 个在 DPC-Exos 和 DPCs 之间差异显著表达的 miRNA，并且前 34 个差异表达 miRNA 的预测靶基因表明 DPC-Exos 通过参与细胞信号转导、脂肪酸表达调节和细胞通讯的基因来调节 HFSCs 的增殖和分化。miR-22-5p 的过表达表明它负调节 HFSC 的增殖，并且被揭示为 miR-22-5p 的直接靶基因。因此，我们提出 miR-22-5p 轴作为调节 HFSC 增殖的新途径。
