College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian, 271018, PR China.
Environ Toxicol Pharmacol. 2018 Jun;60:37-44. doi: 10.1016/j.etap.2018.04.008. Epub 2018 Apr 8.
In this paper, model soil organism, earthworms (Eisenia fetida), were selected to examine the chronic toxic effect of two different ionic liquids (ILs) [Omim]BF and [Omim]Br. Earthworms were put into different ILs concentrations (0, 5, 10, 20, 40 mg/kg) in artificial soil and random selected on days 7, 14, 21 and 28. Reactive oxygen species (ROS), antioxidant enzymes and detoxifying enzyme glutathione-S-transferase (GST) were researched for determination of antioxidant stress. Malondialdehyde (MDA) and olive tail moment (OTM) were researched to determine the oxidative damage. Both the pollutants had the same effect on earthworms: ILs led to accumulation of ROS, and then antioxidant enzymes and detoxification enzyme all changed to eliminate the effects of ROS, and the above process led to lipid peroxidation and DNA damage in earthworms. This paper shows that [Omim]BF and [Omim]Br both caused toxicity to earthworms and had the similar toxicity levels.
本研究选用模式土壤生物蚯蚓（赤子爱胜蚓），考察了两种不同离子液体（ILs）[Omim]BF 和 [Omim]Br 的慢性毒性效应。将蚯蚓分别置于不同 ILs 浓度（0、5、10、20、40mg/kg）的人工土壤中，在第 7、14、21 和 28 天随机选择。研究了活性氧（ROS）、抗氧化酶和解毒酶谷胱甘肽-S-转移酶（GST），以确定抗氧化应激。研究了丙二醛（MDA）和橄榄尾矩（OTM），以确定氧化损伤。两种污染物对蚯蚓的影响相同：ILs 导致 ROS 积累，然后抗氧化酶和解毒酶都发生变化以消除 ROS 的影响，这一过程导致蚯蚓的脂质过氧化和 DNA 损伤。本文表明，[Omim]BF 和 [Omim]Br 均对蚯蚓产生毒性，且毒性水平相似。
