CAS Key Laboratory of Mechanical Behavior and Design of Material, Department of Modern Mechanics, University of Science and Technology of China, Hefei, 230027, China.
CAS Key Laboratory of Mechanical Behavior and Design of Material, Department of Modern Mechanics, University of Science and Technology of China, Hefei, 230027, China.
Anal Chim Acta. 2018 Aug 22;1020:116-122. doi: 10.1016/j.aca.2018.02.072. Epub 2018 Mar 10.
Profenofos, a highly poisonous organophosphorus pesticide, has been widely used in agricultural production. These pesticide residues have seriously influenced food security and threatened human health, and new methods with high sensitivity are greatly needed to detect profenofos. Here, we developed an aptamer-based microcantilever-array sensor operated in stress mode to detect profenofos, with advantages of being a label-free, highly sensitive, one-step immobilization method capable of quantitative and real-time detection. The microcantilevers were functionalized with a profenofos-specific aptamer (SS2-55), and then the specific binding of profenofos to aptamer induced a deflection of the microcantilever, which was monitored using an optical method in a real-time manner. The microcantilever deflection showed a positive relationship with profenofos concentration, and the detection limit was low to 1.3 ng mL (3.5 nM) for profenofos, which was much lower than other aptamer-based detection methods. The selectivity of the sensor was verified with another organophosphorus pesticide. Additionally, we successfully detected profenofos dissolved in vegetable-soak solution. Our results showed that this aptamer-based microcantilever-array sensor is a convenient and label-free method for detecting profenofos in small amounts and has great potential for food-security applications.
丙溴磷是一种高毒性有机磷农药，已被广泛应用于农业生产中。这些农药残留严重影响了食品安全，威胁到人类健康，因此急需开发高灵敏度的新方法来检测丙溴磷。在这里，我们开发了一种基于适配体的微悬臂梁阵列传感器，该传感器以应变为工作模式，用于检测丙溴磷，具有无需标记、灵敏度高、一步固定化、可定量和实时检测等优点。微悬臂梁用丙溴磷特异性适配体（SS2-55）功能化，然后丙溴磷与适配体的特异性结合诱导微悬臂梁的偏转而通过光学方法实时监测。微悬臂梁的偏转角与丙溴磷浓度呈正相关，检测限低至 1.3ng/mL（3.5nM），远低于其他基于适配体的检测方法。传感器的选择性通过另一种有机磷农药进行了验证。此外，我们还成功检测了蔬菜浸泡液中的丙溴磷。我们的结果表明，这种基于适配体的微悬臂梁阵列传感器是一种方便、无标记的检测痕量丙溴磷的方法，在食品安全应用方面具有很大的潜力。
