State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Biosens Bioelectron. 2016 May 15;79:353-8. doi: 10.1016/j.bios.2015.12.060. Epub 2015 Dec 21.
Liver cancer is one of the most common and highly malignant cancers in the world. There are no effective therapeutic options if an early liver cancer diagnosis is not achieved. In this work, detection of HepG2 cells by label-free microcantilever array aptasensor was developed. The sensing microcantilevers were functionalized by HepG2 cells-specific aptamers. Meanwhile, to eliminate the interferences induced by the environment, the reference microcantilevers were modified with 6-mercapto-1-hexanol self-assembled monolayers. The aptasensor exhibits high specificity over not only human liver normal cells, but also other cancer cells of breast, bladder, and cervix tumors. The linear relation ranges from 1×10(3) to 1×10(5)cells/mL, with a detection limit of 300 cells/mL (S/N=3). Our work provides a simple method for detection of liver cancer cells with advantages in terms of simplicity and stability.
肝癌是世界上最常见和高度恶性的癌症之一。如果不能早期诊断肝癌，就没有有效的治疗选择。在这项工作中，开发了无标记微悬臂阵列适体传感器对 HepG2 细胞的检测。传感微悬臂通过 HepG2 细胞特异性适体功能化。同时，为了消除环境引起的干扰，参考微悬臂用 6-巯基-1-己醇自组装单层修饰。该适体传感器不仅对人肝正常细胞，而且对乳腺癌、膀胱癌和宫颈癌等其他癌细胞均表现出高特异性。线性关系范围从 1×10(3)到 1×10(5)个细胞/mL，检测限为 300 个细胞/mL(S/N=3)。我们的工作为肝癌细胞的检测提供了一种简单的方法，具有简单和稳定的优点。
