Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Shanghai Agricultural Product Quality and Safety Center (Shanghai), Shanghai 200125, China.
Biosensors (Basel). 2023 Feb 22;13(3):309. doi: 10.3390/bios13030309.
Chlorpyrifos (CHL), profenofos (PRO) and cypermethrin (CYP) are widely used in combination to increase crop yields. However, these three pesticides can cause serious harm to human health and do not easily degrade. In this study, a novel visible paper sensor has been prepared successfully and different colorimetric reactions were utilized to detect the three pesticides simultaneously. The sensor was constructed by grafting a zwitterionic polymer onto a cellulose filter (CF) and placing it on a glass surface modified with PDMS. The branch shape was designed to form multiple detection areas, which were modified with specific pesticides and corresponding chromogenic reagents. The as-prepared colorimetric platform exhibited high sensitivity, a short detection time, a good linear response and a low detection limit (LOD) for the three pesticides (chlorpyrifos: y = 46.801 - 1.939x, R = 0.983, LOD = 0.235 mg/L; profenofos: y = 40.068 + 42.5x, R = 0.988, LOD = 4.891 mg/L; cypermethrin: y = 51.993 + 1.474x, R = 0.993, LOD = 4.053 mg/L). The comparison of the results obtained by the proposed paper sensor and those obtained by spectrophotometry further revealed the stability and reliability of the paper sensor. In particular, the color intensity of the interaction between the pesticides and coloring agents could be directly observed by the human eye. The consistency of the colorimetric/optical assay was proven in real target pesticide samples. Thus, this sensing strategy provides a portable, cost-effective, accurate and visualized paper platform, which could be suitable for application in the fruit and vegetable industry for monitoring CHL, PRO and CYP in parallel.
毒死蜱（CHL）、丙溴磷（PRO）和氯氰菊酯（CYP）广泛用于联合提高作物产量。然而，这三种农药会对人类健康造成严重危害，而且不易降解。在本研究中，成功制备了一种新型可见纸传感器，并利用不同的比色反应同时检测这三种农药。该传感器通过将两性离子聚合物接枝到纤维素滤纸上（CF），并将其放置在经过 PDMS 修饰的玻璃表面上构建而成。设计的支链形状形成了多个检测区域，这些区域分别用特定的农药和相应的显色试剂进行了修饰。所制备的比色平台对这三种农药表现出高灵敏度、短检测时间、良好的线性响应和低检测限（LOD）（毒死蜱：y = 46.801 - 1.939x，R = 0.983，LOD = 0.235 mg/L；丙溴磷：y = 40.068 + 42.5x，R = 0.988，LOD = 4.891 mg/L；氯氰菊酯：y = 51.993 + 1.474x，R = 0.993，LOD = 4.053 mg/L）。与分光光度法相比，提出的纸传感器的结果比较进一步证明了纸传感器的稳定性和可靠性。特别是，可以直接用肉眼观察到农药与显色剂相互作用的颜色强度。在实际目标农药样品中证明了比色/光学测定的一致性。因此，这种传感策略提供了一种便携、经济高效、准确和可视化的纸平台，可适用于果蔬行业中同时监测 CHL、PRO 和 CYP。
