Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Food Chem. 2018 Aug 30;258:174-180. doi: 10.1016/j.foodchem.2018.03.036. Epub 2018 Mar 9.
Small collagen peptides are associated with various benefits, such as bone and skin health. However, preparation of small collagen peptides from terrestrial vertebrate origins remains a challenge. Here, we show that pretreatment with formic acid enhanced the production of small collagen peptides from spent hen skin. After pretreatment with formic acid, the percentage of small peptides below 2 kDa increased to 48.92% and 43.34% from 33.79% and 36.32% for heat-soluble collagen (HSC) and pepsin-soluble collagen (PSC), respectively. Pretreatment with formic acid degraded telopeptides and released the cross-links (pyrrole and pyridinoline), which made hen collagen more susceptible to papain hydrolysis. LC-MS/MS results revealed that none of the peptides identified from HSC-FA (formic acid)-Papain and PSC-FA-Papain were derived from cross-linked telopeptides. These results demonstrated that formic acid assisted the hydrolysis of highly cross-linked collagen of spent hens, and it might also be used to produce small collagen peptides from other aged, vertebrate collagens.
小胶原肽与多种益处相关，如骨骼和皮肤健康。然而，从陆生脊椎动物来源制备小胶原肽仍然是一个挑战。在这里，我们表明甲酸预处理增强了从废弃鸡皮中生产小胶原肽的能力。经过甲酸预处理后，小于 2 kDa 的小肽的比例分别从热溶性胶原蛋白（HSC）和胃蛋白酶溶性胶原蛋白（PSC）的 33.79%和 36.32%增加到 48.92%和 43.34%。甲酸预处理会降解端肽并释放交联物（吡咯和吡啶啉），使鸡胶原蛋白更易受木瓜蛋白酶水解。LC-MS/MS 结果表明，从 HSC-FA（甲酸）-木瓜蛋白酶和 PSC-FA-木瓜蛋白酶中鉴定出的肽都不是来自交联的端肽。这些结果表明，甲酸辅助了废弃鸡中高度交联胶原蛋白的水解，并且它也可用于从其他老化的脊椎动物胶原蛋白中生产小胶原肽。
