Oxlund H, Jørgensen P H, Ortoft G, Andreassen T T
Department of Connective Tissue Biology, University of Aarhus, Denmark.
Connect Tissue Res. 1991;26(1-2):65-75. doi: 10.3109/03008209109152164.
The effect of biosynthetic human growth hormone (b-hGH) treatment on rat skin collagen was investigated. Groups of rats were injected with b-hGH 0.16, 1.10 and 8.33 mg/kg/day for 90 days. The weight gain of the rats treated with b-hGH 1.10 and 8.33 mg/kg/day was 13% and 82% higher, respectively, compared with that of the placebo control group. The extractability of the skin collagen was studied by extraction with phosphate buffered saline (pH 7.4), followed by acetic acid (0.5 M) and acetic acid with pepsin. The reducible collagen cross-links were measured after reduction of the cross-links by KB3H4, followed by acid hydrolysis and ion-exchange chromatography. Furthermore, patterns of cyanogen bromide peptides were studied by SDS-poly-acrylamide-gel-electrophoresis. Peptides bound together by stable cross-links and the relative amounts of collagen type I and collagen type III were measured. Treatment with b-hGH 8.33 mg/kg/day resulted in increased extractability of the skin collagen in acetic acid, increased relative amounts of reducible collagen cross-links and reduced amounts of high molecular weight cyanogen bromide cleaved peptides of the collagen. These alterations probably reflect an increased synthesis of skin collagen induced by the highest dose of b-hGH. The relative amounts of collagen type I and collagen type III of the skin were not influenced by the b-hGH treatment.
研究了生物合成人生长激素（b-hGH）治疗对大鼠皮肤胶原蛋白的影响。将大鼠分组，分别注射0.16、1.10和8.33mg/kg/天的b-hGH，持续90天。与安慰剂对照组相比，接受1.10和8.33mg/kg/天b-hGH治疗的大鼠体重增加分别高出13%和82%。通过用磷酸盐缓冲盐水（pH 7.4）、随后用乙酸（0.5M）以及乙酸与胃蛋白酶进行提取来研究皮肤胶原蛋白的可提取性。在用KB3H4还原交联后，通过酸水解和离子交换色谱法测量可还原的胶原蛋白交联。此外，通过SDS-聚丙烯酰胺凝胶电泳研究溴化氰肽的模式。测量通过稳定交联结合在一起的肽以及I型和III型胶原蛋白的相对含量。8.33mg/kg/天的b-hGH治疗导致皮肤胶原蛋白在乙酸中的可提取性增加、可还原的胶原蛋白交联相对含量增加以及胶原蛋白的高分子量溴化氰裂解肽含量减少。这些改变可能反映了最高剂量的b-hGH诱导的皮肤胶原蛋白合成增加。皮肤中I型和III型胶原蛋白的相对含量不受b-hGH治疗的影响。
