Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Food Chem. 2018 Aug 30;258:51-58. doi: 10.1016/j.foodchem.2018.03.050. Epub 2018 Mar 13.
Iron(III) ethylenediaminetetraacetate (Fe(III)EDTA) is widely used in iron fortification for reducing iron deficiency, and its determination is urgently needed. The present work developed a fluorescent method to straightforwardly determine Fe(III)EDTA by using photoluminescent carbon dots (C-dots) with the aid of F ions as the masking agent of free Fe ions. In the presence of F ions, only Fe(III)EDTA selectively quenched the photoluminescence of C-dots, and both Fe and Fe as well as other carboxylic acids have no effect on the emission of C-dots. The sensing mechanism was attributed to the ligand-tailored electron transfer process from C-dots to Fe. Under optimum conditions, this method showed a linear calibration plot over the Fe(III)EDTA range of 1.0-200 μmol L and a detection limit of 0.4 μmol L. The proposed method was successfully applied to determine Fe(III)EDTA in real samples with acceptable recoveries of spikes (95%-110%) and repeatability (RSD, 4.2%-9.5%).
三价铁乙二胺四乙酸盐（Fe(III)EDTA）广泛用于铁强化以减少缺铁，因此迫切需要对其进行测定。本工作开发了一种荧光法，通过使用荧光碳点（C-dots）并借助 F 离子作为游离 Fe 离子的掩蔽剂，直接测定 Fe(III)EDTA。在 F 离子存在下，只有 Fe(III)EDTA 选择性猝灭 C-dots 的光致发光，而 Fe 和 Fe 以及其他羧酸对 C-dots 的发射没有影响。传感机制归因于从 C-dots 到 Fe 的配体剪裁电子转移过程。在最佳条件下，该方法在 1.0-200 μmol·L 的 Fe(III)EDTA 范围内呈现线性校准曲线，检测限为 0.4 μmol·L。该方法成功应用于实际样品中 Fe(III)EDTA 的测定，加标回收率为 95%-110%，重复性（RSD，4.2%-9.5%）可接受。
