Ye Hongli, Zhao Lukai, Ren Xinghui, Cai Youqiong, Chi Hai
Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China.
Key Laboratory of Control of Safety and Quality for Aquatic Product, Ministry of Agriculture and Rural Affairs, Beijing 100141, China.
Nanomaterials (Basel). 2022 Jan 10;12(2):213. doi: 10.3390/nano12020213.
An approach to the detection of F ions in food samples was developed based on a "switch-off-on" fluorescence probe of silicon nanoparticles (SiNPs). The fluorescence of the synthetic SiNPs was gradually quenched in the presence of Fe ion and slightly recovered with the addition of F ion owing to the formation of a stable and colorless ferric fluoride. The fluorescence recovery exhibited a good linear relationship (R = 0.9992) as the concentration of F ion increased from 0 to 100 μmol·L. The detection limit of the established method of F ion was 0.05 μmol·L. The recovery experiments confirmed the accuracy and reliability of the proposed method. The ultraviolet-visible spectra, fluorescence decays, and zeta potentials evidenced the fluorescence quenching mechanism involving the electron transfer between the SiNPs and Fe ion, while the fluorescence recovery resulted from the formation of ferric fluoride. Finally, SiNPs were successfully applied to detect F ions in tap water, Antarctic krill, and Antarctic krill powder.
基于硅纳米颗粒（SiNPs）的“关-开”荧光探针，开发了一种检测食品样品中氟离子的方法。在铁离子存在下，合成的SiNPs的荧光逐渐猝灭，由于形成了稳定且无色的氟化铁，加入氟离子后荧光略有恢复。随着氟离子浓度从0增加到100 μmol·L，荧光恢复呈现出良好的线性关系（R = 0.9992）。所建立的氟离子检测方法的检测限为0.05 μmol·L。回收率实验证实了该方法的准确性和可靠性。紫外可见光谱、荧光衰减和zeta电位证明了荧光猝灭机制涉及SiNPs与铁离子之间的电子转移，而荧光恢复是由氟化铁的形成引起的。最后，SiNPs成功应用于检测自来水、南极磷虾和南极磷虾粉中的氟离子。
