Oxidative Stress and Cell Signaling Research Group, Centro Interdisciplinar de Pesquisas em Biotecnologia - CIPBIOTEC, Universidade Federal do Pampa, Campus São Gabriel, RS, Brazil; Departamento de Bioquímica e Biologia Molecular, CCNE, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
Oxidative Stress and Cell Signaling Research Group, Centro Interdisciplinar de Pesquisas em Biotecnologia - CIPBIOTEC, Universidade Federal do Pampa, Campus São Gabriel, RS, Brazil.
Free Radic Biol Med. 2018 May 20;120:395-406. doi: 10.1016/j.freeradbiomed.2018.04.549. Epub 2018 Apr 12.
Many studies have shown the effects of sleep deprivation in several aspects of health and disease. However, little is known about how mitochondrial bioenergetics function is affected under this condition. To clarify this, we developed a simple model of short-term sleep deprivation, in which fruit-flies were submitted to a nocturnal light condition and then mitochondrial parameters were assessed by high resolution respirometry (HRR). Exposure of flies to constant light was able to alter sleep patterns, causing locomotor deficits, increasing ROS production and lipid peroxidation, affecting mitochondrial activity, antioxidant defense enzymes and caspase activity. HRR analysis showed that sleep deprivation affected mitochondrial bioenergetics capacity, decreasing respiration at oxidative phosphorylation (OXPHOS) and electron transport system (ETS). In addition, the expression of genes involved in the response to oxidative stress and apoptosis were increased. Thus, our results suggest a connection between sleep deprivation and oxidative stress, pointing to mitochondria as a possible target of this relationship.
许多研究表明，睡眠剥夺会对健康和疾病的多个方面产生影响。然而，对于在这种情况下线粒体生物能量学功能如何受到影响，人们知之甚少。为了阐明这一点，我们建立了一个简单的短期睡眠剥夺模型，其中果蝇被置于夜间光照条件下，然后通过高分辨率呼吸测定法（HRR）评估线粒体参数。将果蝇暴露于恒定光线下能够改变睡眠模式，导致运动缺陷，增加 ROS 产生和脂质过氧化，影响线粒体活性、抗氧化防御酶和半胱天冬酶活性。HRR 分析表明，睡眠剥夺会影响线粒体生物能量学能力，降低氧化磷酸化（OXPHOS）和电子传递系统（ETS）的呼吸作用。此外，与氧化应激和细胞凋亡反应相关的基因表达增加。因此，我们的结果表明睡眠剥夺与氧化应激之间存在联系，提示线粒体可能是这种关系的一个潜在靶点。
