Aisenberg A C, Wilkes B M, Jacobson J O
Department of Medicine, Massachusetts General Hospital, Boston 02114.
Blood. 1988 Apr;71(4):969-72.
Southern blotting was used to detect rearrangement of the bcl-2 gene in 104 cases of non-Hodgkin's lymphoma subclassified by the Working Formulation, 24 cases of B cell chronic lymphocytic leukemia (B-CLL) and 14 cases of T cell malignancy. Earlier workers reported rearrangement of this gene (located on chromosome 18) in a major fraction of follicular lymphomas, lymphomas in which a 14;18 chromosome translocation is frequently observed. In the present study, bcl-2 was rearranged in 30% (11 of 37) of follicular lymphomas and 19% (11 of 58) of diffuse lymphomas of follicle center cell lineage. In 18 of 19 samples studied, the rearranged bcl-2 fragment also hybridized with a probe for the joining region of the immunoglobulin heavy chain gene located on chromosome 14, indicating a 14;18 translocation. In lymphomas not derived from follicle center cells, ie, diffuse lymphomas of small B lymphocytes, B-CLL and T cell neoplasms, the bcl-2 gene was always in germline configuration. The frequent rearrangement of bcl-2 in a variety of B cell lymphomas of diffuse morphology (small cleaved cell, large cell, small noncleaved cell and immunoblastic) is noteworthy.
采用Southern印迹法检测了按照工作分类法分类的104例非霍奇金淋巴瘤、24例B细胞慢性淋巴细胞白血病（B-CLL）和14例T细胞恶性肿瘤中bcl-2基因的重排情况。早期研究人员报道，在大部分滤泡性淋巴瘤中该基因（位于18号染色体）发生重排，而在这类淋巴瘤中经常观察到14;18染色体易位。在本研究中，30%（37例中的11例）的滤泡性淋巴瘤和19%（58例中的11例）的滤泡中心细胞系弥漫性淋巴瘤中bcl-2发生重排。在研究的19个样本中的18个中，重排的bcl-2片段也与位于14号染色体上的免疫球蛋白重链基因连接区的探针杂交，表明存在14;18易位。在非滤泡中心细胞来源的淋巴瘤中，即小B淋巴细胞弥漫性淋巴瘤、B-CLL和T细胞肿瘤中，bcl-2基因总是处于种系构型。值得注意的是，在各种弥漫性形态的B细胞淋巴瘤（小裂细胞型、大细胞型、小无裂细胞型和免疫母细胞型）中bcl-2频繁重排。
