DePasquale C, Leri J
Department of Biology, Pennsylvania State University - Altoona, Altoona, PA, USA.
Department of Biology, Pennsylvania State University - Altoona, Altoona, PA, USA.
Behav Processes. 2018 Dec;157:638-644. doi: 10.1016/j.beproc.2018.04.006. Epub 2018 Apr 12.
In non-human mammals, exercise has been shown to decrease anxiety-like behavior. Conversely, a number of studies have reported no effect or even an increase in anxiety-like behavior after exercise, however, inconsistent training regimes and behavioral paradigms across studies may be confounding the results. Zebrafish (Danio rerio) are a well-established animal model in neurobehavioral research, and have the potential to shed new insight into the effects of exercise on anxiety-like behavior where previous research has been limited, due to the ability to precisely control intensity and duration of exercise, and the validation of tests for measuring different aspects of anxiety-like behaviors. In the current study, fish were split between two treatment groups; Exercised and Control. Fish in the exercised condition were aerobically challenged (max water velocity: 0.5 m/s) using a swim tunnel one hour a day, five days a week, for six weeks. Control fish spent an equal amount of time in the swim tunnel but were not aerobically challenged (max water velocity: 0.05 m/s). After six weeks, all fish were tested individually in two standard complimentary anxiety tests for zebrafish: the novel tank test and the light-dark test. Exercised fish exhibited reduced anxiety-like behaviors in the novel tank test; they spent more time in the top and were quicker to enter the top of a novel tank compared to Control fish. In addition, Exercised fish spent more time in the light compartment of the light-dark test compared to Control fish. Our results demonstrate the beneficial effect of exercise on anxiety-like behavior in zebrafish.
在非人类哺乳动物中，运动已被证明可减少类似焦虑的行为。相反，一些研究报告称运动后对类似焦虑的行为没有影响，甚至有所增加，然而，各研究中不一致的训练方案和行为范式可能会混淆研究结果。斑马鱼（Danio rerio）是神经行为研究中一种成熟的动物模型，由于能够精确控制运动强度和持续时间，以及用于测量类似焦虑行为不同方面的测试的有效性，它有可能为以往研究受限的运动对类似焦虑行为的影响提供新的见解。在本研究中，将鱼分为两个治疗组：运动组和对照组。运动组的鱼每天使用游泳隧道进行一小时的有氧挑战（最大水流速度：0.5米/秒），每周五天，持续六周。对照鱼在游泳隧道中花费相同的时间，但不进行有氧挑战（最大水流速度：0.05米/秒）。六周后，所有鱼分别在两项针对斑马鱼的标准补充焦虑测试中进行测试：新水箱测试和明暗测试。在新水箱测试中，运动组的鱼表现出较少的类似焦虑行为；与对照鱼相比，它们在水箱上部花费更多时间，并且更快进入新水箱的上部。此外，与对照鱼相比，运动组的鱼在明暗测试的亮区花费更多时间。我们的结果证明了运动对斑马鱼类似焦虑行为的有益影响。
