Drug Discovery and Development Division, Patanjali Research Institute, Haridwar 249 401, India.
Department of Allied and Applied Sciences, University of Patanjali, Patanjali Yog Peeth, Haridwar 249 401, India.
Biomolecules. 2020 Apr 13;10(4):600. doi: 10.3390/biom10040600.
Zebrafish is an elegant vertebrate employed to model the pathological etiologies of human maladies such as cardiac diseases. Persistent physiological stresses can induce abnormalities in heart functions such as cardiac hypertrophy (CH), which can lead to morbidity and mortality. In the present study, using zebrafish as a study model, efficacy of the traditional Indian Ayurveda medicine "Yogendra Ras" (YDR) was validated in ameliorating drug-induced cardiac hypertrophy. YDR was prepared using traditionally described methods and composed of nano- and micron-sized metal particles. Elemental composition analysis of YDR showed the presence of mainly Au, Sn, and Hg. Cardiac hypertrophy was induced in the zebrafish following a pretreatment with erythromycin (ERY), and the onset and reconciliation of disease by YDR were determined using a treadmill electrocardiogram, heart anatomy analysis, C-reactive protein release, and platelet aggregation time-analysis. YDR treatment of CH-induced zebrafish showed comparable results with the Standard-of-care drug, verapamil, tested in parallel. Under in-vitro conditions, treatment of isoproterenol (ISP)-stimulated murine cardiomyocytes (H9C2) with YDR resulted in the suppression of drug-stimulated biomarkers of oxidative stress: COX-2, NOX-2, NOX-4, ANF, troponin-I, -T, and cardiolipin. Taken together, zebrafish showed a strong disposition as a model for studying the efficacy of Ayurvedic medicines towards drug-induced cardiopathies. YDR provided strong evidence for its capability in modulating drug-induced CH through the restoration of redox homeostasis and exhibited potential as a viable complementary therapy.
斑马鱼是一种优雅的脊椎动物，可用于模拟人类疾病（如心脏病）的病理病因。持续的生理应激会导致心脏功能异常，如心肌肥厚（CH），从而导致发病率和死亡率。在本研究中，使用斑马鱼作为研究模型，验证了传统印度阿育吠陀药物“Yogendra Ras”（YDR）在改善药物诱导的心肌肥厚方面的疗效。YDR 是按照传统描述的方法制备的，由纳米和微米级的金属颗粒组成。YDR 的元素组成分析表明，主要含有 Au、Sn 和 Hg。在用红霉素（ERY）预处理后，在斑马鱼中诱导心肌肥厚，并通过跑步机心电图、心脏解剖分析、C 反应蛋白释放和血小板聚集时间分析来确定 YDR 对疾病的起始和缓解作用。YDR 治疗 CH 诱导的斑马鱼的结果与平行测试的标准护理药物维拉帕米相当。在体外条件下，YDR 处理异丙肾上腺素（ISP）刺激的鼠心肌细胞（H9C2）可抑制药物刺激的氧化应激生物标志物：COX-2、NOX-2、NOX-4、ANF、肌钙蛋白-I、-T 和心磷脂。综上所述，斑马鱼作为研究阿育吠陀药物对药物诱导性心脏病疗效的模型具有很强的优势。YDR 为其通过恢复氧化还原平衡来调节药物诱导的 CH 的能力提供了有力证据，并显示出作为可行的补充治疗方法的潜力。
