El-Ashram Saeed, Li Cuiping, Abouhajer Fathi, Mehmood Rashid, Al Nasr Ibrahim, Zhang Yinghui, Lu Tang, Yili Ding, Suo Xun, Haoji Zhang, Li Zhili, Huang Shujian
College of Life Science and Engineering, Foshan University, 18 Jiangwan Street, Foshan 528231, Guangdong Province, China; State Key Laboratory for Agrobiotechnology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Faculty of Science, Kafr ElSheikh University, Kafr ElSheikh, Egypt.
College of Life Science and Engineering, Foshan University, 18 Jiangwan Street, Foshan 528231, Guangdong Province, China.
Vet Parasitol. 2018 Apr 30;254:105-113. doi: 10.1016/j.vetpar.2018.02.042. Epub 2018 Mar 1.
We have set up an ex vivo ovine abomasal model, which can mimic the multicellular process to explore the early steps in haemonchine nematode infection using RNA-seq technology. Ovine abomasal explants were collected for histological and transcriptional analysis and supernatants collected to quantitate lactate dehydrogenase (LDH) enzymes. Atotal of 233 were substantially induced genes between L-inoculated and uninoculated-control tissues, respectively. However, a total of 14 were considerably down-regulated genes between the 51 aforementioned tissues. Fifteen pathways were annotated by Kyoto Encyclopedia of Genes, and Genomes pathway analysis accounted for the significant percentage in immediate response to larval-stage of H. contortus. Key genes upregulated in response to the addition of L-inoculum of H. contortus were IL-6, IL-8, C1q, Atypical chemokine receptor-3, chemokine ligand-2, manganese superoxide dismutase, integrin alpha-7, -8, -9, integrin subunit beta-1, integrin subunit beta 6, intercellular adhesion molecule-1 and actin alpha-1. This study shows for the first time that galectin-1 is up-regulated in an ex vivo abomasal segment model exposed to L-inoculum of H. contortus following 6 h of incubation. The abomasal segment model has been shown to be a suitable tool to study the haemonchine larval-stage effects on the ovine abomasal tissues prior to in vivo assessment.
我们建立了一种体外绵羊皱胃模型，该模型可以模拟多细胞过程，利用RNA测序技术探索血矛线虫感染的早期步骤。收集绵羊皱胃外植体进行组织学和转录分析，并收集上清液以定量乳酸脱氢酶（LDH）。在接种L3幼虫和未接种对照组织之间，分别共有233个基因被显著诱导。然而，在上述51个组织之间共有14个基因被显著下调。通过京都基因与基因组百科全书注释了15条通路，基因组通路分析显示在对捻转血矛线虫幼虫阶段的即时反应中占显著比例。在添加捻转血矛线虫L3接种物后上调的关键基因包括IL-6、IL-8、C1q、非典型趋化因子受体-3、趋化因子配体-2、锰超氧化物歧化酶、整合素α-7、-8、-9、整合素亚基β-1、整合素亚基β6、细胞间粘附分子-1和肌动蛋白α-1。本研究首次表明，在体外皱胃节段模型中，经6小时孵育后，暴露于捻转血矛线虫L3接种物时半乳糖凝集素-1会上调。在进行体内评估之前，皱胃节段模型已被证明是研究血矛线虫幼虫阶段对绵羊皱胃组织影响的合适工具。
