Watts Michelle E, Williams Sarah M, Nithianantharajah Jess, Claudianos Charles
Queensland Brain Institute, The University of Queensland, Brisbane QLD 4072, Australia.
Monash Bioinformatics Platform, Monash University, Melbourne VIC 3800, Australia.
Noncoding RNA. 2018 Mar 27;4(2):10. doi: 10.3390/ncrna4020010.
Hypoxia-regulated microRNA-210 (miR-210) is a highly conserved microRNA, known to regulate various processes under hypoxic conditions. Previously we found that miR-210 is also involved in honeybee learning and memory, raising the questions of how neural activity may induce hypoxia-regulated genes and how miR-210 may regulate plasticity in more complex mammalian systems. Using a pull-down approach, we identified 620 unique target genes of miR-210 in humans, among which there was a significant enrichment of age-related neurodegenerative pathways, including Huntington's, Alzheimer's, and Parkinson's diseases. We have also validated that miR-210 directly regulates various identified target genes of interest involved with neuronal plasticity, neurodegenerative diseases, and miR-210-associated cancers. This data suggests a potentially novel mechanism for how metabolic changes may couple plasticity to neuronal activity through hypoxia-regulated genes such as miR-210.
缺氧调节的微小RNA-210（miR-210）是一种高度保守的微小RNA，已知其在缺氧条件下调节各种过程。此前我们发现miR-210也参与蜜蜂的学习和记忆，这就引出了神经活动如何诱导缺氧调节基因以及miR-210如何在更复杂的哺乳动物系统中调节可塑性的问题。使用下拉法，我们在人类中鉴定出了620个miR-210的独特靶基因，其中与年龄相关的神经退行性疾病途径显著富集，包括亨廷顿舞蹈症、阿尔茨海默病和帕金森病。我们还验证了miR-210直接调节各种已鉴定的与神经元可塑性、神经退行性疾病以及与miR-210相关癌症有关的靶基因。这些数据表明了一种潜在的新机制，即代谢变化如何通过miR-210等缺氧调节基因将可塑性与神经元活动联系起来。
