Effect of lipopolysaccharide derived from surabaya isolates on alveolar bone destruction.

BACKGROUND

lipopolysaccharide (LPS) has a high virulence factor. It interacts with serum protein through receptors on the epithelial cell surface, thereby increasing both interleukin (IL)-1β, and IL-6 which results in damage to periodontal tissue.

AIM

The aim of the study was to identify and evaluate the effect of LPS derived from local isolates ( on the destruction of alveolar bone by means of several biomarkers, including; the number of osteoblasts and osteoclasts, the expression of IL-6, matrix metallopeptidase 1 (MMP-1), and receptor activator of nuclear factor kappa-Β ligand (RANKL).

MATERIALS AND METHODS

The isolation of LPS from was calculated using phenol, while purification was performed using Sephadex C-18 column chromatography. 40 Wistar rats were divided into four groups of 10. Each treatment was divided into two groups which were 0.9% NaCl and LPS induced for 7 and 14 days, respectively. Gingival and alveolar bones were further introduced into the induction area, followed by the measuring of osteoblast and osteoclast with hematoxylin-eosin staining, IL-6, MMP-1 and RANKL expression with immunohistochemical.

RESULTS

Reduced numbers of osteoblasts at the 7 and 14 day of treatment were detected, while those of osteoclasts increased. There was an increased expression of IL-6, MMP-1, and RANKL in the 7 and 14-day treatment group. Treatment of LPS from over 7 and 14 days resulted in damage to periodontal tissue and alveolar bone in Wistar rats.

CONCLUSION

LPS of administration for 7 and 14 days causes periodontal and alveolar tissue destruction in Wistar rats.