Janus M M, Crielaard W, Volgenant C M C, van der Veen M H, Brandt B W, Krom B P
Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands; Top Institute Food and Nutrition, Wageningen, The Netherlands.
Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam , Amsterdam , The Netherlands.
J Oral Microbiol. 2017 Jan 23;9(1):1270613. doi: 10.1080/20002297.2016.1270613. eCollection 2017.
The yeast is an oral commensal microorganism, occurring in the oral cavity of 50-70% of healthy individuals. Its effect on oral ecology has mostly been studied using dual-species models, which disregards the complex nature of oral biofilms. The aim of this study was to culture in a complex model to study its effect on oral biofilms. Biofilms, inoculated using pooled stimulated saliva with or without addition of , were grown under anaerobic, aerobic, or aerobic +5% CO conditions. Red autofluorescence was quantified using a spectrophotometer and visualized in fluorescence photographs. The microbiome of 5 h biofilms was determined using 16S rDNA sequencing. was only able to proliferate in biofilms grown under aerobic conditions. After 48 h, did not induce differences in total biofilm formation, lactic acid accumulation (cariogenic phenotype) or protease activity (periodontitis phenotype). , anaerobically grown biofilms developed red autofluorescence, irrespective of inoculum. However, under aerobic conditions, only -containing biofilms showed red autofluorescence. Facultative or strict anaerobic , and genera were significantly more abundant in biofilms with . Biofilms without contained more of the aerobic and facultative anaerobic genera , and . The presence of alters the bacterial microbiome in early oral biofilms, resulting in the presence of strictly anaerobic bacteria under oxygen-rich conditions. This study illustrates that should not be disregarded in healthy oral ecosystems, as it has the potential to influence bacteria significantly.
酵母是一种口腔共生微生物，在50%-70%的健康个体口腔中存在。其对口腔生态的影响大多是使用双物种模型进行研究的，而这种模型忽略了口腔生物膜的复杂性质。本研究的目的是在一个复杂模型中培养酵母，以研究其对口腔生物膜的影响。使用添加或不添加酵母的混合刺激唾液接种生物膜，在厌氧、需氧或需氧+5%二氧化碳条件下培养。使用分光光度计对红色自发荧光进行定量，并在荧光照片中可视化。使用16S rDNA测序确定5小时生物膜的微生物群。酵母仅能在需氧条件下生长的生物膜中增殖。48小时后，酵母未诱导总生物膜形成、乳酸积累（致龋表型）或蛋白酶活性（牙周炎表型）出现差异。无论接种物如何，厌氧培养的生物膜都会产生红色自发荧光。然而，在需氧条件下，只有含酵母的生物膜显示出红色自发荧光。在含有酵母的生物膜中，兼性或严格厌氧的梭杆菌属和普氏菌属明显更为丰富。不含酵母的生物膜含有更多的需氧和兼性厌氧属，如放线菌属和罗氏菌属。酵母的存在会改变早期酵母口腔生物膜中的细菌微生物群，导致在富氧条件下存在严格厌氧菌。这项研究表明，在健康的口腔生态系统中，酵母不应被忽视，因为它有可能对细菌产生重大影响。
