Liu James X, Bravo Dalibel, Buza John, Kirsch Thorsten, Kennedy Oran, Rokito Andrew, Zuckerman Joseph D, Virk Mandeep S
New York University Langone Medical Center, Hospital for Joint Diseases, Departments of Orthopaedic Surgery and Cell Biology, New York, NY, United States.
J Orthop. 2018 Jan 30;15(1):53-58. doi: 10.1016/j.jor.2018.01.032. eCollection 2018 Mar.
The purpose of this study was to examine the influence of topical vancomycin on cell migration and survival of tissue healing cells. Human osteoblasts, myoblasts and fibroblasts were exposed to vancomycin at concentrations of 1, 3, 6, or 12 mg/cm for either a 1-h or 48-h (continuous) duration. Continuous exposure to all vancomycin concentrations significantly reduced cell survival (<22% cells survived) and migration in osteoblasts and myoblasts (P < 0.001). 1-h vancomycin exposure reduced osteoblast and myoblast survival and migration only at 12 mg/cm (P < 0.001). Further studies are warranted to optimize the dosage of intrawound vancomycin.
本研究的目的是检验局部应用万古霉素对组织愈合细胞迁移和存活的影响。将人成骨细胞、成肌细胞和成纤维细胞暴露于浓度为1、3、6或12mg/cm²的万古霉素中，持续1小时或48小时（连续）。持续暴露于所有万古霉素浓度下均显著降低了成骨细胞和成肌细胞的细胞存活率（<22%的细胞存活）以及迁移能力（P<0.001）。仅在12mg/cm²浓度下，1小时的万古霉素暴露降低了成骨细胞和成肌细胞的存活率及迁移能力（P<0.001）。有必要进行进一步研究以优化伤口内万古霉素的剂量。
