Roller Brandon L, Stoker Aaron M, Cook James L
Wake Forest School of Medicine, Department of Radiology, Winston-Salem, NC, USA.
University of Missouri, Department of Orthopaedic Surgery, Columbia, MO, USA.
J Clin Orthop Trauma. 2020 Oct;11(Suppl 5):S729-S734. doi: 10.1016/j.jcot.2020.06.011. Epub 2020 Jun 17.
This study tests the elution capabilities of a magnesium phosphate cement (MPC). Study objectives were to quantify the passive release of magnesium ions from MPC and to assess the effects of antibiotic-loaded MPC on bacterial growth and osteoblast viability.
MPC constructs were created and incubated in fetal bovine serum (FBS). At 2, 4, and 17 weeks, a sample was collected for magnesium ion concentration analysis. Control and vancomycin-loaded (vanc) MPC beads were also created. Zone of inhibition was measured after incubating beads on agar plates for 24 h. Osteoblasts were seeded onto control and vanc beads and cultured for 9 days. Metabolic activity was measured via a resazurin assay. ANOVA with Tukey HSD post-hoc tests and t-tests were performed.
Magnesium ions were eluted at 2 and 4-week time points without significant difference, but demonstrated a significant spike at the 17-week time point. Zones of inhibition for the bacterial species was observed for Vanc-MPC beads, but not control beads. No cytotoxic effects on osteoblasts were noted.
MPC has potential to improve bone regeneration based on its ability to passively elute magnesium. Additionally, antibiotic-loaded MPC inhibits bacterial growth while avoiding osteoblast cytotoxicity.
本研究测试磷酸镁骨水泥（MPC）的洗脱能力。研究目标是量化MPC中镁离子的被动释放，并评估载抗生素MPC对细菌生长和成骨细胞活力的影响。
制备MPC构建体并在胎牛血清（FBS）中孵育。在第2、4和17周时，采集样本进行镁离子浓度分析。还制备了对照和载万古霉素（vanc）的MPC微珠。将微珠在琼脂平板上孵育24小时后测量抑菌圈。将成骨细胞接种到对照和vanc微珠上并培养9天。通过刃天青测定法测量代谢活性。进行了带有Tukey HSD事后检验的方差分析和t检验。
镁离子在第2周和第4周时间点被洗脱，无显著差异，但在第17周时间点出现显著峰值。观察到Vanc-MPC微珠对细菌有抑菌圈，而对照微珠没有。未观察到对成骨细胞的细胞毒性作用。
基于其被动洗脱镁的能力，MPC有改善骨再生的潜力。此外，载抗生素的MPC抑制细菌生长，同时避免成骨细胞的细胞毒性。
