Yang Mingying, Shuai Yajun, Sunderland Kegan S, Mao Chuanbin
Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China.
Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019-5300, USA.
Adv Funct Mater. 2017 Nov 24;27(44). doi: 10.1002/adfm.201703726. Epub 2017 Oct 5.
Little is known about the role of biocompatible protein nanoridges in directing stem cell fate and tissue regeneration due to the difficulty in forming protein nanoridges. Here an ice-templating approach is proposed to produce semi-parallel pure silk protein nanoridges. The key to this approach is that water droplets formed in the protein films are frozen into ice crystals (removed later by sublimation), pushing the surrounding protein molecules to be assembled into nanoridges. Unlike the flat protein films, the unique protein nanoridges can induce the differentiation of human mesenchymal stem cells (MSCs) into osteoblasts without any additional inducers, as well as the formation of bone tissue in a subcutaneous rat model even when not seeded with MSCs. Moreover, the nanoridged films induce less inflammatory infiltration than the flat films in vivo. This work indicates that decorating biomaterials surfaces with protein nanoridges can enhance bone tissue formation in bone repair.
由于形成蛋白质纳米脊存在困难，目前对于生物相容性蛋白质纳米脊在引导干细胞命运和组织再生方面的作用知之甚少。在此，我们提出一种冰模板法来制备半平行的纯丝蛋白纳米脊。该方法的关键在于，蛋白质薄膜中形成的水滴会冻结成冰晶（随后通过升华去除），促使周围的蛋白质分子组装成纳米脊。与平坦的蛋白质薄膜不同，独特的蛋白质纳米脊能够在没有任何额外诱导剂的情况下诱导人间充质干细胞（MSC）分化为成骨细胞，并且即使在未接种MSC的情况下，也能在大鼠皮下模型中形成骨组织。此外，在体内，纳米脊薄膜比平坦薄膜诱导的炎症浸润更少。这项工作表明，用蛋白质纳米脊修饰生物材料表面可以增强骨修复中的骨组织形成。
