一种从慢性活动性EB病毒感染患者中建立NK细胞系和T细胞系的高效简便方法。
An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection.
作者信息
Xu Chongfeng, Ai Junhong, Zhang Qingxun, Li Ting, Wu Xiaorong, Xie Zhengde, Duan Ziyuan
机构信息
Genetic Resources Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences.
MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University.
出版信息
J Vis Exp. 2018 Mar 30(133):56515. doi: 10.3791/56515.
A number of methods have been described to establish NK/T cell lines from patients with lymphoma or lymphoproliferative syndrome. These methods employed feeder cells, purified NK or T cells with as much as 10 mL of blood, or a high-dose of IL-2. This study presents a new method with a powerful and simple strategy to establish NK and T cell lines by culturing the peripheral blood mononuclear cells (PBMC) with the addition of recombinant human IL-2 (rhIL-2), and uses as little as 2 mL of whole blood. The cells can proliferate quickly in two weeks and be maintained for more than 3 months. With this method, 7 NK or T cell lines have been established with a high success rate. This method is simple, reliable, and applicable to establishing cell lines from more cases of CAEBV or NK/T cell lymphoma.
已经描述了多种从淋巴瘤或淋巴增殖综合征患者中建立NK/T细胞系的方法。这些方法采用饲养细胞、用多达10毫升血液纯化的NK或T细胞，或高剂量的白细胞介素-2。本研究提出了一种新方法，该方法采用强大且简单的策略，通过添加重组人白细胞介素-2（rhIL-2）培养外周血单个核细胞（PBMC）来建立NK和T细胞系，并且仅使用2毫升全血。细胞可在两周内快速增殖并维持3个月以上。用这种方法已成功建立了7个NK或T细胞系。该方法简单、可靠，适用于从更多慢性活动性EB病毒感染（CAEBV）或NK/T细胞淋巴瘤病例中建立细胞系。
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