State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
J Anim Sci. 2018 Jun 4;96(6):2342-2351. doi: 10.1093/jas/sky129.
The objective of this study was to investigate the effects of Lactobacillus reuteri LR1, a new strain isolated from the feces of weaned pigs, on the growth performance, intestinal morphology, immune responses, and intestinal barrier function in weaned pigs. A total of 144 weaned pigs (Duroc × Landrace × Yorkshire, 21 d of age) with an initial BW of 6.49 ± 0.02 kg were randomly assigned to 3 dietary treatments with 8 replicate pens, each of per treatment and 6 pigs. Pigs were fed a basal diet (CON, controls), the basal diet supplemented with 100 mg/kg olaquindox and 75 mg/kg aureomycin (OA) or the basal diet supplemented with 5 × 1010 cfu/kg L. reuteri LR1 for a 14-d period. At the end of study, the ADG, ADFI, and G:F were calculated, and 1 randomly selected pig from each pen was euthanized for sample collection. The LR1 increased ADG (22.73%, P < 0.05) compared with CON. The villus height of the ileum was increased (P < 0.05) and crypt depth in duodenum was reduced (P < 0.05), along with increased (P < 0.05) villus height to crypt depth ratio of the jejunum and ileum by LR1 compared with CON and OA. LR1 increased (P < 0.05) ileal mucosal content of IL-22 and transforming growth factor-β compared with OA. Compared with CON, LR1 increased (P < 0.05) and OA decreased (P < 0.05) the ileal content of secretory immunoglobulin A (sIgA), and the abundance of transcripts of porcine β-defensin 2 and protegrin 1-5. Compared with CON, LR1 increased (P < 0.05) tight junction protein zonula occludens-1 and occludin transcripts in the mucosa of the jejunum and ileum, and those of mucin-2 in ileal mucosa. The relative expression of toll-like receptor 2 (TLR2) and TLR4 were increased (P < 0.05) in ileal mucosa in pigs fed LR1 compared with CON. In conclusion, these data indicated that dietary LR1 supplementation at 5 × 1010 cfu/kg improved growth performance, intestinal morphology, and intestinal barrier function in weaned pigs.
本研究旨在探究从断奶仔猪粪便中分离出的新菌株乳杆菌 LR1 对断奶仔猪生长性能、肠道形态、免疫应答和肠道屏障功能的影响。将 144 头 21 日龄、初始体重为 6.49±0.02kg 的杜洛克×长白×约克夏断奶仔猪随机分为 3 个处理组，每组 8 个重复，每个重复 6 头猪。对照组饲喂基础日粮，基础日粮中添加 100mg/kg 喹乙醇和 75mg/kg 金霉素（OA），试验组在基础日粮中添加 5×1010cfu/kg 乳杆菌 LR1，试验期为 14d。试验结束时，计算平均日增重（ADG）、平均日采食量（ADFI）和料重比（G:F），每个重复栏随机选择 1 头猪进行采样。与对照组相比，LR1 组 ADG 提高了 22.73%（P<0.05）。LR1 组十二指肠隐窝深度降低（P<0.05），空肠绒毛高度增加（P<0.05），空肠和回肠的绒毛高度与隐窝深度的比值增加（P<0.05）。与 OA 组相比，LR1 组回肠黏膜中白细胞介素-22（IL-22）和转化生长因子-β的含量增加（P<0.05）。与对照组相比，LR1 组和 OA 组的回肠黏膜分泌型免疫球蛋白 A（sIgA）含量增加（P<0.05），仔猪防御素 2 和防御素 1-5 的转录物含量降低（P<0.05）。与对照组相比，LR1 组空肠和回肠黏膜中紧密连接蛋白闭合蛋白-1 和闭合蛋白的转录物增加（P<0.05），回肠黏膜中黏蛋白-2 的转录物增加（P<0.05）。LR1 组回肠黏膜中 Toll 样受体 2（TLR2）和 TLR4 的相对表达量增加（P<0.05）。综上所述，日粮中添加 5×1010cfu/kg 的乳杆菌 LR1 可改善断奶仔猪的生长性能、肠道形态和肠道屏障功能。
