Tang Qingsong, Yi Hongbo, Hong Weibin, Wu Qiwen, Yang Xuefen, Hu Shenglan, Xiong Yunxia, Wang Li, Jiang Zongyong
State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Key Laboratory of Animal Breeding and Nutrition, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
College of Animal Science, Institute of Animal Nutrition and Feed Science, Guizhou University, Guiyang, China.
Front Vet Sci. 2021 Nov 11;8:728849. doi: 10.3389/fvets.2021.728849. eCollection 2021.
CGMCC 1258 and LR1 are two important strains of probiotics. However, their different advantages in the probiotic effect of weaned pigs are still poorly understood. Therefore, the study was to investigate the comparative effects of dietary supplementation of CGMCC 1258 and LR1 on growth performance, antioxidant function, and intestinal immunity in weaned pigs. Ninety barrows [initial body weight (BW) = 6.10 ± 0.1 kg] 21 days old were randomly divided into 3 treatments with 5 replicates, each replicate containing 6 pigs. Pigs in control (CON) were fed a basal diet, and the basal diets supplemented with 5 × 10 CFU/kg CGMCC 1258 (LP) or LR1 (LR) for 42 days, respectively. The results showed that LP increased ( < 0.05) serum superoxide dismutase (SOD), and decreased ( < 0.05) serum malondialdehyde (MDA) and the expression and secretion of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) in intestinal mucosa, but has no significant effect on growth performance and diarrheal incidence. However, LR increased ( < 0.05) final BW and average daily gain (ADG), reduced ( < 0.05) 29-42-day diarrheal incidence, decreased ( < 0.05) the expression and secretion of IL-1β, IL-6, TNF-α, and IFN-γ, and increased ( < 0.05) the expression of transforming growth factor-β (TGF-β) in intestinal mucosa. In addition, the serum glutathione peroxidase (GSH-PX), mRNA relative expression of Na+-K+-2Cl- co-transporter 1 (NKCC1) and cystic fibrosis transmembrane conductance regulator (CFTR) and the content of toll-like relative (TLR2) and TLR4 in the jejunum, and secretory immunoglobulin (sIgA) content of ileal mucosa were higher ( < 0.05) than LP. Collectively, dietary CGMCC 1258 improved intestinal morphology, intestinal permeability, intestinal immunity, and antioxidant function in weaned pigs. Dietary LR1 showed better growth performance, a lower incidence of diarrhea, better intestinal morphology, and a higher extent of immune activation in weaned pigs.
中国普通微生物菌种保藏管理中心1258株和LR1株是两种重要的益生菌菌株。然而，它们在断奶仔猪益生菌作用方面的不同优势仍未得到充分了解。因此，本研究旨在探讨日粮中添加中国普通微生物菌种保藏管理中心1258株和LR1株对断奶仔猪生长性能、抗氧化功能和肠道免疫的比较影响。选取90头21日龄的公猪（初始体重=6.10±0.1千克），随机分为3组，每组5个重复，每个重复6头猪。对照组（CON）饲喂基础日粮，基础日粮分别添加5×10⁸CFU/千克中国普通微生物菌种保藏管理中心1258株（LP）或LR1株（LR），持续42天。结果表明，LP组血清超氧化物歧化酶（SOD）升高（P<0.05），血清丙二醛（MDA）以及肠道黏膜中白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）的表达和分泌降低（P<0.05），但对生长性能和腹泻发生率无显著影响。然而，LR组最终体重和平均日增重（ADG）增加（P<0.05），29至42日龄腹泻发生率降低（P<0.05），IL-1β、IL-6、TNF-α和IFN-γ的表达和分泌降低（P<0.05），肠道黏膜中转化生长因子-β（TGF-β）的表达增加（P<0.05）。此外，血清谷胱甘肽过氧化物酶（GSH-PX）、空肠中Na⁺-K⁺-2Cl⁻共转运体1（NKCC1）和囊性纤维化跨膜传导调节因子（CFTR）的mRNA相对表达以及Toll样受体（TLR2）和TLR4的含量，以及回肠黏膜分泌型免疫球蛋白（sIgA）含量均高于LP组（P<0.05）。总体而言，日粮添加中国普通微生物菌种保藏管理中心1258株可改善断奶仔猪的肠道形态、肠道通透性、肠道免疫和抗氧化功能。日粮添加LR1株在断奶仔猪中表现出更好的生长性能、更低的腹泻发生率、更好的肠道形态和更高的免疫激活程度。
