Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Egypt.
Biochemistry Department, Faculty of Science, Menoufia University, Egypt.
Arch Biochem Biophys. 2018 Jun 1;647:41-46. doi: 10.1016/j.abb.2018.04.009. Epub 2018 Apr 14.
Colorectal cancer (CRC) is a common cancer worldwide that affects men and women of all racial and ethnic groups. Recent evidence supports the role of microRNAs in CRC. We planned to investigate microRNA200c expression and its relation with diagnosis, prognosis, metastasis and overall survival in CRC patients. This study enrolled 90 subjects (3'0 CRC patients, 30 patients with benign colorectal polyps and 30 healthy control subjects).
Laboratory investigations included measurement of serum CA19-9 and CEA by enzyme linked immunosorbent assay (ELISA) method and relative quantitation (RQ) of microRNA200c gene expression by real time PCR technique.
Significant higher MicroRNA200c expression levels in CRC patients versus both benign (P < 0.011) and control groups (P < 0.001), additionally, benign group had elevated levels versus control (P < 0.001). MicroRNA 200c at cutoff >4.56 had sensitivity 86.67% and specificity 73.33% (P < 0.001) for CRC discrimination. Kaplan-Meier survival analysis revealed significant association (P = 0.028) of high expression of microRNA200c with decreased overall survival.
Noticeable up-regulation of microRNA200c in CRC and its remarkable relation with unfavorable survival suggesting its potential dual use as a diagnostic and prognostic biomarker for CRC.
结直肠癌（CRC）是一种常见的癌症，影响着所有种族和民族的男性和女性。最近的证据支持 microRNA 在 CRC 中的作用。我们计划研究 microRNA200c 的表达及其与 CRC 患者的诊断、预后、转移和总生存期的关系。本研究纳入了 90 名受试者（30 名 CRC 患者、30 名良性结直肠息肉患者和 30 名健康对照者）。
实验室研究包括通过酶联免疫吸附测定（ELISA）法测量血清 CA19-9 和 CEA，以及通过实时 PCR 技术相对定量（RQ）microRNA200c 基因表达。
CRC 患者的 MicroRNA200c 表达水平明显高于良性（P < 0.011）和对照组（P < 0.001），此外，良性组的水平也高于对照组（P < 0.001）。MicroRNA 200c 截断值>4.56 对 CRC 具有 86.67%的敏感性和 73.33%的特异性（P < 0.001）。Kaplan-Meier 生存分析显示，高表达 MicroRNA200c 与总生存期缩短显著相关（P = 0.028）。
CRC 中 microRNA200c 的显著上调及其与不良生存的显著关系表明，其具有作为 CRC 诊断和预后生物标志物的双重潜力。
