miRNA-92a 通过靶向糖尿病中的 KLF2 来保护胰岛 B 细胞功能。
MiRNA-92a protects pancreatic B-cell function by targeting KLF2 in diabetes mellitus.
机构信息
International Medical Center, Tianjin First Central Hospital, Tianjin, China.
Department of Pharmacy, Tianjin First Central Hospital, Tianjin, China.
出版信息
Biochem Biophys Res Commun. 2018 Jun 7;500(3):577-582. doi: 10.1016/j.bbrc.2018.04.097.
AIMS
diabetes mellitus is one of the most common metabolic diseases worldwide characterized by insulin resistance and pancreatic β cell dysfunction. miRNA plays an important role in DM. In previous studies, miRNA-92a could function as targets for innovative precision medicines to reduce T1D islet autoimmunity. However, the relationship between miRNA-92a and pancreatic β cell dysfunction remains unknown. The aim of the study was to investigate the role of miRNA-92a in pancreatic β cell dysfunction.
METHODS
Apoptosis, proliferation, insulin secretion and cell survival rate were detected to evaluate the function of miRNA-92a.
RESULTS
we found that miRNA-92a could inhibit apoptosis induced by high-glucose environment and increase the insulin secretion and proliferation. Moreover, we identify the KLF2 as direct target of miRNA-92a, suggesting that miRNA-92a may function through regulating KLF2.
CONCLUSION
Altogether, we verified the function and mechanism of miRNA-92a and provide evidence that miRNA-92a may serve a potential candidate for the clinical treatment for DM.
目的
糖尿病是一种全球范围内最常见的代谢疾病，其特征为胰岛素抵抗和胰腺β细胞功能障碍。miRNA 在糖尿病中发挥重要作用。在之前的研究中，miRNA-92a 可以作为创新精准药物的靶点，以减少 1 型糖尿病胰岛自身免疫。然而，miRNA-92a 与胰腺β细胞功能障碍之间的关系尚不清楚。本研究旨在探讨 miRNA-92a 在胰腺β细胞功能障碍中的作用。
方法
通过检测细胞凋亡、增殖、胰岛素分泌和细胞存活率来评估 miRNA-92a 的功能。
结果
我们发现 miRNA-92a 可抑制高糖环境诱导的细胞凋亡，并增加胰岛素分泌和增殖。此外，我们确定 KLF2 是 miRNA-92a 的直接靶标，表明 miRNA-92a 可能通过调节 KLF2 发挥作用。
结论
总之，我们验证了 miRNA-92a 的功能和机制，并提供了证据表明 miRNA-92a 可能成为治疗糖尿病的潜在候选药物。
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