Peto Katalin, Nemeth Norbert, Mester Anita, Magyar Zsuzsanna, Ghanem Souleiman, Somogyi Viktoria, Tanczos Bence, Deak Adam, Bidiga Laszlo, Frecska Ede, Nemes Balazs
Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Department of Pathology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Clin Hemorheol Microcirc. 2018;70(1):107-117. doi: 10.3233/CH-170361.
Micro-rheological relations of renal ischemia-reperfusion (I/R) have not been completely elucidated yet. Concerning anti-inflammatory agents, it is supposed that sigma-1 receptor agonist N,N-dimethyl-tryptamin (DMT) can be useful to reduce I/R injury.
To investigate the micro-rheological and metabolic parameters, and the effects of DMT in renal I/R in rats.
In anesthetized rats from median laparotomy both kidneys were exposed. In Control group (n = 6) no other intervention happened. In I/R group (n = 10) the right renal vessels were ligated and after 60 minutes the organ was removed. The left renal vessels were clamped for 60 minutes followed by 120-minute reperfusion. In I/R+DMT group (n = 10) DMT was administered 15 minutes before the ischemia. Blood samples were taken before/after ischemia and during the reperfusion for testing hematological, metabolic parameters, erythrocyte deformability and aggregation.
Lactate concentration significantly increased and accompanied with decreased blood pH. Enhanced erythrocyte aggregation and impaired deformability were observed from the 30th minute of reperfusion. In I/R+DMT group we found diminished changes compared to the I/R group (lactate, pH, electrolytes, red blood cell deformability and aggregation).
Metabolic and micro-rheological parameters impair during renal I/R. DMT could reduce but not completely prevent the changes in this rat model.
肾脏缺血再灌注（I/R）的微观流变学关系尚未完全阐明。关于抗炎药物，推测σ-1受体激动剂N，N-二甲基色胺（DMT）可能有助于减轻I/R损伤。
研究大鼠肾脏I/R过程中的微观流变学和代谢参数，以及DMT的作用。
对经正中剖腹术麻醉的大鼠暴露双侧肾脏。对照组（n = 6）未进行其他干预。I/R组（n = 10）结扎右侧肾血管，60分钟后切除该器官。夹闭左侧肾血管60分钟，然后再灌注120分钟。I/R+DMT组（n = 10）在缺血前15分钟给予DMT。在缺血前后及再灌注期间采集血样，检测血液学、代谢参数、红细胞变形性和聚集性。
乳酸浓度显著升高，并伴有血液pH值降低。再灌注第30分钟起观察到红细胞聚集增强和变形性受损。与I/R组相比，I/R+DMT组的变化（乳酸、pH值、电解质、红细胞变形性和聚集性）有所减轻。
肾脏I/R过程中代谢和微观流变学参数受损。在该大鼠模型中，DMT可减轻但不能完全阻止这些变化。
