Department of Clinical Laboratory, Ningbo Municipal Hospital of Traditional Chinese Medicine, Affiliated to Zhejiang Chinese Medical University, Ningbo, 315000, People's Republic of China.
Department of Endocrinology, Ningbo Municipal Hospital of Traditional Chinese Medicine, Affiliated to Zhejiang Chinese Medical University, Ningbo, 315000, People's Republic of China.
BMC Complement Altern Med. 2019 Jul 30;19(1):193. doi: 10.1186/s12906-019-2598-1.
Wenshen Jianpi recipe (WSJPR), a blended traditional Chinese medicine, is considered to have the possible beneficial effect on the progression of diabetic nephropathy (DN). This present study was designed to elucidate this protective activity in a rat model with streptozotocin (STZ)-induced DN and to explore the possible underlying mechanism.
Adult Sprague Dawley (SD) rats were induced to develop DN through intraperitoneal injection of STZ (60 mg/kg). Animals were orally administered saline, WSJPR at 7.5, 15, 30 g/kg, and valsartan (25 mg/kg) daily for 8 weeks. Blood and 24-h urine samples of each rat were collected for biochemical examination at 2-week intervals. Microcirculatory blood flow in the renal cortex and hemorheology index were also measured. At the end of 8 weeks, all rats were sacrificed to obtain the kidney tissues for histological examination and reverse transcription polymerase chain reaction (RT-PCR) was used to analyze the transcriptional levels of nephrin and podocin genes.
WSJPR could improve serum total protein (TP) and albumin (ALB), reduce the excretion rates of urine-TP (U-TP), urine-ALB (U-ALB) and urine urea nitrogen (UUN) (P < 0.05), although it did not significantly alter the hyperglycemia. In addition, treatment with WSJPR could strongly reduce blood flow, erythrocyte aggregation index, and ameliorate microcirculation. In histological measurement, WSJPR-treated rats showed a significant amelioration in glomerular hypertrophy and mesangial expansion. By RT-PCR, we found WSJPR up-regulated the nephrin and podocin expression at mRNA levels.
This study suggested that WSJPR could effectively relieve renal damage and improve renal function of DN rats by ameliorating metabolism disorder and increasing the gene expression of nephrin and podocin, which might be a useful approach for the treatment of DN.
稳神健脾方（WSJPR）是一种中药复方，被认为对糖尿病肾病（DN）的进展可能有有益作用。本研究旨在通过链脲佐菌素（STZ）诱导的 DN 大鼠模型阐明这种保护活性，并探讨其可能的作用机制。
成年 Sprague Dawley（SD）大鼠经腹腔注射 STZ（60mg/kg）诱导产生 DN。动物每天口服给予生理盐水、WSJPR （7.5、15、30g/kg）和缬沙坦（25mg/kg），共 8 周。每 2 周收集一次大鼠的血液和 24 小时尿液样本，用于生化检查。还测量了肾皮质的微循环血流和血液流变学指标。8 周结束时，所有大鼠被处死以获取肾脏组织进行组织学检查，并通过逆转录聚合酶链反应（RT-PCR）分析nephrin 和 podocin 基因的转录水平。
WSJPR 可提高血清总蛋白（TP）和白蛋白（ALB），降低尿总蛋白（U-TP）、尿白蛋白（U-ALB）和尿尿素氮（UUN）的排泄率（P<0.05），尽管它对高血糖没有明显影响。此外，WSJPR 治疗可显著降低血流、红细胞聚集指数，改善微循环。在组织学测量中，WSJPR 治疗的大鼠肾小球肥大和系膜扩张明显改善。通过 RT-PCR，我们发现 WSJPR 在 mRNA 水平上上调了 nephrin 和 podocin 的表达。
本研究表明，WSJPR 通过改善代谢紊乱和增加 nephrin 和 podocin 的基因表达，可有效缓解 DN 大鼠的肾损伤并改善肾功能，可能是治疗 DN 的一种有效方法。
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