Hasegawa Junichi, Yanaihara Atsushi, Iwasaki Shinji, Negishi Momoko, Suzuki Norio, Kawamura Toshihiro, Okai Takashi
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo and.
Denentoshi Ladies Clinic, Yokohama, Japan.
Reprod Med Biol. 2006 Nov 23;5(4):277-282. doi: 10.1111/j.1447-0578.2006.00153.x. eCollection 2006 Dec.
Steroid levels have been used as the predictive parameters for oocyte maturation and embryo development. In the present study, estradiol and progesterone concentrations in the follicular fluid and serum were evaluated in conventional fertilization (IVF; follicle stimulating hormone [FSH] and/or human menopausal gonadotropin [hMG] after pituitary desensitization) and friendly IVF (no stimulation, clomiphene citrate, small dose of FSH or hMG without pituitary desensitization). The purpose of the present study was to evaluate the differences in steroid distribution between conventional and friendly IVF. Concentrations of estradiol, progesterone, FSH, and luteinizing hormone (LH) in serum and follicular fluid were determined in conventional and friendly IVF protocols by an enzyme-linked immunosorbent assay kit. Correlations between follicular fluid and serum steroid concentrations in these different protocols, and between pregnant cycles and steroid concentrations were evaluated. Two hundred and thirty-four samples of follicular fluid from 74 IVF patients were analyzed. In conventional IVF, there was no relationship in steroid levels in between follicular fluid and serum steroids, whereas serum steroid concentrations correlated with the number of developing follicles. There was a relationship between the serum and follicular fluid estradiol levels ( = 0.467, < 0.0001) as well as progesterone levels ( = 0.227, = 0.0488) from friendly IVF patients. Serum steroid concentrations were mainly associated with the number of developing follicles. In the cases of friendly IVF, which had a small number of developing follicles, serum steroids might be used to monitor follicular fluid steroid concentrations. (Reprod Med Biol 2006; : 277-282).
类固醇水平已被用作卵母细胞成熟和胚胎发育的预测参数。在本研究中，对常规体外受精（IVF；垂体脱敏后使用促卵泡激素 [FSH] 和/或人绝经期促性腺激素 [hMG]）和友好IVF（无刺激、克罗米芬柠檬酸盐、小剂量FSH或hMG且无垂体脱敏）患者卵泡液和血清中的雌二醇和孕酮浓度进行了评估。本研究的目的是评估常规IVF和友好IVF之间类固醇分布的差异。通过酶联免疫吸附测定试剂盒测定常规和友好IVF方案中血清和卵泡液中雌二醇、孕酮、FSH和黄体生成素（LH）的浓度。评估了这些不同方案中卵泡液和血清类固醇浓度之间以及妊娠周期与类固醇浓度之间的相关性。对74例IVF患者的234份卵泡液样本进行了分析。在常规IVF中，卵泡液和血清类固醇的水平之间没有关系，而血清类固醇浓度与发育卵泡的数量相关。友好IVF患者的血清和卵泡液雌二醇水平（r = 0.467，P < 0.0001）以及孕酮水平（r = 0.227，P = 0.0488）之间存在相关性。血清类固醇浓度主要与发育卵泡的数量相关。在发育卵泡数量较少的友好IVF病例中，血清类固醇可用于监测卵泡液类固醇浓度。（《生殖医学与生物学》2006年；：277 - 282）
