Hasegawa Junichi, Iwasaki Shinji, Yanaihara Atsushi, Negishi Momoko, Tahara Ryuzo, Okai Takashi
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Reprod Med Biol. 2004 Jan 29;2(4):171-176. doi: 10.1111/j.1447-0578.2003.00043.x. eCollection 2003 Dec.
Several parameters of early embryo development are known as predictors of implantation success. Recently, zygote or embryo morphological assessments are thought to be a major method of selection in embryo transfer. We expected that the concentrations of the steroids in follicular fluid (FF) were associated with oocyte maturation and embryo quality. In the present paper, we evaluated the relationship of several parameters. We investigated 105 samples of FF from 22 subjects by fertilization (IVF). We evaluated the correlations between the FF concentrations of estradiol (E) and progesterone (P), the diameter of the ovarian follicles, fertilization, and zygote assessment based on pronuclear morphology and day 3 embryo qualities (i.e. number of blastomeres and fragmentation rate). There was a positive correlation between the E concentrations in FF and serum ( = 0.273, < 0.01), but there was no correlation between follicular diameter and the FF concentration of each steroid. The concentration of E in FF containing fertilized oocytes was not significantly different from that in FF containing unfertilized oocytes. At the pronuclear stage, the concentration of either steroid in FF did not differ among the morphological groups. The concentration of P in FF was significantly lower in the group in which pronuclei were detected at 20 h after insemination than in the group in which pronuclei were not detected. The concentration of E in FF was significantly related to the number of blastomeres ( = 0.271, < 0.05) and furthermore, was significantly higher in FF from which morphologically good embryos were obtained at day 3 ( < 0.05). The FF concentrations of the steroids did not affect the pronuclear pattern, but P production may play a role in reducing the potential of the oocyte to develop pronuclei and the concentration of E may predict the cleavage capability of the oocyte. (Reprod Med Biol 2003; : 171-176).
早期胚胎发育的几个参数被认为是着床成功的预测指标。最近，合子或胚胎形态学评估被认为是胚胎移植中主要的选择方法。我们预期卵泡液（FF）中类固醇的浓度与卵母细胞成熟和胚胎质量相关。在本文中，我们评估了几个参数之间的关系。我们通过体外受精（IVF）研究了来自22名受试者的105份FF样本。我们评估了FF中雌二醇（E）和孕酮（P）的浓度、卵巢卵泡直径、受精情况以及基于原核形态的合子评估与第3天胚胎质量（即卵裂球数量和碎片率）之间的相关性。FF中E的浓度与血清中E的浓度呈正相关（r = 0.273，P < 0.01），但卵泡直径与每种类固醇的FF浓度之间无相关性。含有受精卵母细胞的FF中E的浓度与含有未受精卵母细胞的FF中E的浓度无显著差异。在原核阶段，FF中任何一种类固醇的浓度在形态学分组之间无差异。授精后20小时检测到原核的组中FF中P的浓度显著低于未检测到原核的组。FF中E的浓度与卵裂球数量显著相关（r = 0.271，P < 0.05），此外，在第3天获得形态良好胚胎的FF中E的浓度显著更高（P < 0.05）。类固醇的FF浓度不影响原核模式，但P的产生可能在降低卵母细胞发育原核的潜能方面起作用，而E的浓度可能预测卵母细胞的分裂能力。（《生殖医学与生物学》2003年；：171 - 176）
