College of Plant Science and Technology, Huazhong Agricultural University, The Provincial Key Laboratory of Plant Pathology of Hubei Province, Wuhan, 430070, China.
College of Plant Protection, China Agricultural University, State Key Laboratory of Agrobiotechnology, Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management, Beijing, 100193, China.
New Phytol. 2018 Aug;219(3):1031-1047. doi: 10.1111/nph.15141. Epub 2018 Apr 16.
Protein post-translational modifications play critical roles in cellular processes, development and stress response. The small ubiquitin-like modifier (SUMO) to proteins is one of the essential modifications in eukaryotes, but its function remains largely unknown in plant pathogenic fungi. We present a comprehensive analysis combined with proteomic, molecular and cellular approaches to explore the roles of sumoylation in the model plant fungal pathogen, Magnaporthe oryzae. We found the SUMO pathway plays key roles in colony growth, conidia formation and virulence to the host, as well as cell-cycle-related phenotypes. Sumoylation is also involved in responding to different stresses. Affinity purification identified 940 putative SUMO substrates, many of which were reported to be involved in development, stress response and infection. Interestingly, four septins were also shown to be sumoylated. Mutation of consensus sumoylation sites in each septin all resulted in reduced virulence to the host and dislocation of septins in appressoria. Moreover, sumoylation is also involved in extracellular secretion of different effector proteins. Our study on the functions of sumoylation provides novel insight into development and infection of the rice blast fungus.
蛋白质翻译后修饰在细胞过程、发育和应激反应中起着关键作用。蛋白质的小泛素样修饰物（SUMO）是真核生物中必不可少的修饰物之一，但在植物病原真菌中，其功能仍知之甚少。我们通过蛋白质组学、分子和细胞方法的综合分析，探索了 SUMO 修饰在模式植物病原真菌稻瘟病菌中的作用。我们发现 SUMO 途径在菌落生长、分生孢子形成和对宿主的毒力以及与细胞周期相关的表型中起着关键作用。SUMO 化还参与了对不同应激的反应。亲和纯化鉴定了 940 种推定的 SUMO 底物，其中许多已被报道参与发育、应激反应和感染。有趣的是，四个隔膜蛋白也被 SUMO 化。每个隔膜蛋白中保守 SUMO 修饰位点的突变都导致对宿主的毒力降低和隔膜蛋白在附着胞中的错位。此外，SUMO 化还参与不同效应蛋白的细胞外分泌。我们对 SUMO 化功能的研究为水稻白叶枯病菌的发育和感染提供了新的见解。
