Kose Katsumi
Institute of Applied Physics, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 3058573, Japan.
Anat Rec (Hoboken). 2018 Jun;301(6):987-997. doi: 10.1002/ar.23787. Epub 2018 Apr 17.
Magnetic resonance (MR) microscopy of chemically fixed human embryos performed in University of Tsukuba since 1999 to 2015 was reviewed. More than 1,000 chemically fixed human embryos stored in the Congenital Anomaly Research Center of Kyoto University were used throughout the MR microscopy project, which was divided into three terms. In the first term (1999-2005), 3D MR images of 1,204 embryo specimens were acquired with 128 × 128 × 256 voxels by a super-parallel MR microscope using a 2.35 T horizontal-bore superconducting magnet. In the second term (2005-2006), 3D MR images of seven embryo specimens were acquired with 256 × 256 × 512 voxels by an MR microscope using a 9.4 T vertical wide-bore superconducting magnet. In the third term (2013-2015), 3D MR images of a Carnegie Stage (CS) 21 specimen were acquired with 512 × 512 × 1024 voxels by an MR microscope using a 4.7 T vertical wide-bore superconducting magnet and nuclear magnetic resonance parameters of a CS23 specimen were measured with 128 × 128 × 256-256 × 256 × 512 voxels by an MR microscope using a 9.4 T vertical narrow-bore superconducting magnet. Based on the results obtained in this project, the author has proposed the future MR microscopy project in which a number of embryo specimens will be imaged with 256 × 256 × 512-512 × 512 × 1024 voxels using a newly designed super-parallel MR microscope. Anat Rec, 301:987-997, 2018. © 2018 Wiley Periodicals, Inc.
回顾了1999年至2015年在筑波大学进行的化学固定人类胚胎的磁共振（MR）显微镜检查。在整个MR显微镜检查项目中，使用了存储在京都大学先天性异常研究中心的1000多个化学固定人类胚胎，该项目分为三个阶段。在第一阶段（1999 - 2005年），使用2.35T水平孔径超导磁体的超并行MR显微镜，以128×128×256体素获取了1204个胚胎标本的3D MR图像。在第二阶段（2005 - 2006年），使用9.4T垂直宽孔径超导磁体的MR显微镜，以256×256×512体素获取了7个胚胎标本的3D MR图像。在第三阶段（2013 - 2015年），使用4.7T垂直宽孔径超导磁体的MR显微镜，以512×512×1024体素获取了一个卡内基阶段（CS）21标本的3D MR图像，并使用9.4T垂直窄孔径超导磁体的MR显微镜，以128×128×256 - 256×256×512体素测量了一个CS23标本的核磁共振参数。基于该项目获得的结果，作者提出了未来的MR显微镜检查项目，其中将使用新设计的超并行MR显微镜，以256×256×512 - 512×512×1024体素对多个胚胎标本进行成像。《解剖学记录》，301:987 - 997，2018年。©2018威利期刊公司。
