State Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry , Nankai University , Tianjin , 300071 , China.
Collaborative Innovation Center of Chemical Science and Engineering , Nankai University , Tianjin , 300071 , China.
Anal Chem. 2018 May 1;90(9):5758-5763. doi: 10.1021/acs.analchem.8b00086. Epub 2018 Apr 23.
Multitarget assay with single detection modality from individual stimulation mode has aroused great attention. Here, we realize multitarget detection with the color change as signal source from single white light-emitting metal-organic frameworks (MOFs) under single wavelength excitation for the first time. The white light-emitting MOFs are prepared by carefully tuning the ratio of Eu, Tb, and Dy ions to obtain trimetal MOFs; 5-boronoisophthalic acid (5-bop) provides additional selectivity as ligand. Based on antenna effect, 5-bop is excited to produce its triplet-state, which sensitizes the Ln ions for the white emission. Thus, all of the fluorescence emission is achieved under single wavelength excitation at 275 nm, while any factors affecting the procedure result in the modulation of emission for diverse wavelengths. The trimetal MOFs display distinctive emission colors after interacting with different targets, including metal ions, anions, small molecule, and even biomolecule as a Lab-on-MOFs system. This system shows higher integration degree and simpler preparation and sensing procedures than other multitarget detection strategies.
从单一刺激模式进行单一检测模式的多目标分析引起了极大的关注。在这里，我们首次实现了单一波长激发下，通过单一白光发射金属有机骨架（MOF）的颜色变化作为信号源进行多目标检测。通过仔细调整 Eu、Tb 和 Dy 离子的比例来制备白光发射 MOF；5-硼基异苯二甲酸（5-bop）作为配体提供额外的选择性。基于天线效应，5-bop 被激发产生三重态，从而敏化 Ln 离子产生白光发射。因此，所有的荧光发射都在 275nm 的单一波长激发下实现，而任何影响该过程的因素都会导致不同波长的发射调制。三金属 MOF 与不同的目标相互作用后显示出独特的发射颜色，包括金属离子、阴离子、小分子甚至生物分子作为 MOF 上的实验室系统。与其他多目标检测策略相比，该系统具有更高的集成度和更简单的制备和传感过程。
