Zhou Liya, Song Zhenfei, Zhou Liwen, Qiu Yongchi, Hu Nan, Hu Yong, Hu Xingming
Department of Orthopedics, Xiangyang Hospital of Traditional Chinese Medicine, Xiangyang,China.
Neurosciences (Riyadh). 2018 Apr;23(2):116-121. doi: 10.17712/nsj.2018.4.20170391.
To investigate the neuroprotective effect of Astragalus injection in a spinal cord ischemia-reperfusion (I/R) injury model.
A total of 27 Sprague Dawley rats were randomly divided into 3 groups: control group (n=3), I/R group (n=12), and Astragalus injection group (Ast group, n=12). Spinal cord ischemia was induced by occlusion of the abdominal aorta above the right renal artery for 32 min. Animals in the Ast group were administered Astragalus injection (6.42 mL/kg) at 30 min before the induction of ischemia. After reperfusion for 8, 12, 24, or 48 hours, the serum neuron-specific enolase (NSE) concentration was measured by enzyme-linked immunosorbent assay (ELISA) and the aquaporin-4 (AQP4) protein level was detected by western blotting.
The pathological changes, as assessed by hematoxylin and eosin (HE) staining, were milder in the spinal cords of the Ast group compared to the I/R group. Enzyme-linked immunosorbent assay demonstrated that the NSE concentration of the Ast group was significantly lower than that of the I/R group (p<0.05). However, the NSE concentrations of the I/R and Ast groups were significantly higher than that of the control group (p=0.05). Additionally, the expression of AQP4 in the Ast group was lower than that of the I/R group at each time point.
These findings indicate that Astragalus injection has a neuroprotective effect in spinal cord I/R injury by decreasing the AQP4 expression.
探讨黄芪注射液对脊髓缺血再灌注（I/R）损伤模型的神经保护作用。
将27只Sprague Dawley大鼠随机分为3组：对照组（n = 3）、I/R组（n = 12）和黄芪注射液组（Ast组，n = 12）。通过阻断右肾动脉上方的腹主动脉32分钟诱导脊髓缺血。Ast组动物在缺血诱导前30分钟给予黄芪注射液（6.42 mL/kg）。再灌注8、12、24或48小时后，采用酶联免疫吸附测定（ELISA）法检测血清神经元特异性烯醇化酶（NSE）浓度，采用蛋白质印迹法检测水通道蛋白4（AQP4）蛋白水平。
苏木精-伊红（HE）染色评估显示，Ast组脊髓的病理变化较I/R组轻。酶联免疫吸附测定表明，Ast组的NSE浓度显著低于I/R组（p < 0.05）。然而，I/R组和Ast组的NSE浓度均显著高于对照组（p = 0.05）。此外，Ast组在每个时间点的AQP4表达均低于I/R组。
这些结果表明，黄芪注射液通过降低AQP4表达对脊髓I/R损伤具有神经保护作用。
