Osiński Maciej, Wirstlein Przemysław, Wender-Ożegowska Ewa, Mikołajczyk Mateusz, Jagodziński Paweł Piotr, Szczepańska Małgorzata
Division of Reproduction, Department of Obstetrics, Gynaecology and Gynaecological Oncology, Poznan, Poland.
Ginekol Pol. 2018;89(3):125-134. doi: 10.5603/GP.a2018.0022.
The development of endometriosis is associated with changes in the expression of genes encoding the 3β-hydroxysteroid dehydrogenase type II (HSD3B2) and 17β-hydroxysteroid dehydrogenase type II (HSD17B2), estrogen receptors 1 (ESR1) and 2 (ESR2) and the androgen receptor (AR). However, little is known about the expression of HSD3B2, HSD17B1, HSD17B2, ESR1 ESR2 and AR during the endometrial phases in eutopic endometrium from infertile women with endometriosis.
Using RT-qPCR analysis, we assessed the expression of the studied genes in the follicular and luteal phases in eutopic endometrium from fertile women (n = 17) and infertile women (n = 35) with endometriosis.
In the mid-follicular eutopic endometrium, we observed a significant increase in HSD3B2 transcript levels in all infertile women with endometriosis (p = 0.003), in infertile women with stage I/II endometriosis (p = 0.008) and in infertile women with stage III/IV endometriosis (p = 0.009) compared to all fertile women. There was a significant increase in ESR1 tran-scripts in all infertile women with endometriosis (p = 0.008) and in infertile women with stage I/II endometriosis (p = 0.019) and in infertile women with stage III/IV endometriosis (p = 0.023) compared to all fertile women. In the mid-luteal eutopic endometrium, we did not observe significant differences in HSD3B2, HSD17B1, HSD17B2, ESR1, ESR2 and AR transcripts between infertile women with endometriosis and fertile women.
Observed significant increase in HSD3B2 and ESR1 transcripts in follicular eutopic endometrium from infer-tile women with endometriosis may be related to abnormal biological effect of E2 in endometrium, further affecting the development of human embryos.
子宫内膜异位症的发生与编码Ⅱ型3β-羟基类固醇脱氢酶（HSD3B2）、Ⅱ型17β-羟基类固醇脱氢酶（HSD17B2）、雌激素受体1（ESR1）和2（ESR2）以及雄激素受体（AR）的基因表达变化有关。然而，关于患有子宫内膜异位症的不孕女性在位内膜在月经周期各阶段中HSD3B2、HSD17B1、HSD17B2、ESR1、ESR2和AR的表达情况，目前所知甚少。
我们采用逆转录定量聚合酶链反应（RT-qPCR）分析，评估了来自有生育能力的女性（n = 17）和患有子宫内膜异位症的不孕女性（n = 35）在位内膜在卵泡期和黄体期所研究基因的表达。
在卵泡中期在位内膜中，与所有有生育能力的女性相比，我们观察到所有患有子宫内膜异位症的不孕女性（p = 0.003）、患有Ⅰ/Ⅱ期子宫内膜异位症的不孕女性（p = 0.008）以及患有Ⅲ/Ⅳ期子宫内膜异位症的不孕女性（p = 0.009）中HSD3B2转录水平显著升高。与所有有生育能力的女性相比，所有患有子宫内膜异位症的不孕女性（p = 0.008）、患有Ⅰ/Ⅱ期子宫内膜异位症的不孕女性（p = 0.019）以及患有Ⅲ/Ⅳ期子宫内膜异位症的不孕女性（p = 0.023）中ESR1转录本显著增加。在黄体中期在位内膜中，我们未观察到患有子宫内膜异位症的不孕女性和有生育能力的女性之间HSD3B2、HSD17B1、HSD17B2、ESR1、ESR2和AR转录本有显著差异。
观察到患有子宫内膜异位症的不孕女性卵泡期在位内膜中HSD3B2和ESR1转录本显著增加，可能与子宫内膜中E2的异常生物学效应有关，进而影响人类胚胎的发育。
