子宫内膜异位症中NF1和RASA1基因表达的评估。
Evaluation of NF1 and RASA1 gene expression in endometriosis.
作者信息
Yarahmadi Ghafour, Dehghanian Mehran, Sandoghsaz Reyhaneh Sadat, Savaee Mohamadreza, Shamsi Farimah, Vahidi Mehrjardi Mohammad Yahya
机构信息
Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
出版信息
Eur J Obstet Gynecol Reprod Biol X. 2022 May 4;15:100152. doi: 10.1016/j.eurox.2022.100152. eCollection 2022 Aug.
BACKGROUND
Endometrios affecting 6-10% of women of reproductive ages around the globe. Important pathways, including the MAPK and PI3K / Akt pathways, have been identified in the disease. The NF1 and RASA1 genes inactivate Ras by their own GTPase activity and controlled the high activity of these pathways.
OBJECTIVE
In this study, we measured NF1 and RASA1 gene expression in the endometrial tissues of patients (eutopic and ectopic tissues) compared to the control samples.
MATERIALS AND METHODS
In our study, tissue samples were collected from 15 patients with endometriosis and 15 healthy women. We used quantitative polymerase chain reaction (qRT-PCR) to measure the NF1 and RASA1 gene expression levels in these samples.
RESULTS
We observed a significant decrease in the expression level of the NF1 gene in both eutopic and ectopic samples of endometriosis patients compared to control samples, while the expression of the RASA1 gene was significantly reduced only in ectopic tissues.
背景
子宫内膜异位症影响全球6%-10%的育龄女性。该疾病中已确定了包括丝裂原活化蛋白激酶（MAPK）和磷脂酰肌醇-3激酶/蛋白激酶B（PI3K/Akt）通路在内的重要信号通路。神经纤维瘤病1型（NF1）基因和RAS p21蛋白激活因子1（RASA1）基因通过自身的GTP酶活性使Ras失活，并控制这些信号通路的高活性。
目的
在本研究中，我们检测了与对照样本相比，患者子宫内膜组织（在位和异位组织）中NF1和RASA1基因的表达情况。
材料与方法
在我们的研究中，收集了15例子宫内膜异位症患者和15名健康女性的组织样本。我们使用定量聚合酶链反应（qRT-PCR）来检测这些样本中NF1和RASA1基因的表达水平。
结果
我们观察到，与对照样本相比，子宫内膜异位症患者的在位和异位样本中NF1基因的表达水平均显著降低，而RASA1基因的表达仅在异位组织中显著降低。
Zotero 插件