Szczepańska Małgorzata, Wirstlein Przemyslaw, Zawadzka Małgorzata, Wender-Ożegowska Ewa, Jagodziński Paweł P
a Department of Obstetrics, Gynecology and Gynecological Oncology, Division of Reproduction , Poznan University of Medical Sciences , Poznan , Poland.
b Department of Biochemistry and Molecular Biology , Poznan University of Medical Sciences , Poznan , Poland.
Gynecol Endocrinol. 2018 Dec;34(12):1084-1090. doi: 10.1080/09513590.2018.1490403. Epub 2018 Aug 22.
Little is known about the differences in ten-eleven translocation 1, 2, and 3 (TET1-3) expression in the endometrial phases in eutopic endometrium from infertile women with endometriosis (IWE) and fertile women without endometriosis (FW). Using RT-qPCR and western blot analysis, we assessed the TET expression in the mid-follicular and mid-luteal phases in eutopic endometrium from IWE (n = 38) and FW (n = 18). Both IWE and FW underwent laparoscopic and histological examinations for endometriosis. In the mid-luteal eutopic endometrium in IWE, compared to that of FW, we found significantly reduced levels of TET1 transcripts and proteins (p = .001 and p = .003, respectively) at the severity stage of I/II (p = .029 and p = .003, respectively) and transcripts only at the severity stage of III/IV (p = .003). In the mid-follicular eutopic endometrium of IWE, compared to that of FW, there was a statistically significant reduction in TET2 transcript levels at the severity stage of III/IV (p = .037). Compared to the mid-follicular endometrium, we found a statistically significant increase in TET3 transcript levels during the mid-luteal phase in the eutopic endometrium of all IWE (p = .034) and in the severity stage of III/IV (p = .025). We observed a change in the expression levels of TET1-3 in the eutopic endometrium of IWE.
关于子宫内膜异位症不孕女性（IWE）和非子宫内膜异位症可育女性（FW）在位内膜不同月经周期中10-11易位蛋白1、2和3（TET1-3）表达的差异，目前所知甚少。我们采用逆转录定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹分析，评估了IWE（n = 38）和FW（n = 18）在位内膜卵泡中期和黄体中期的TET表达。IWE和FW均接受了子宫内膜异位症的腹腔镜检查和组织学检查。在IWE的黄体中期在位内膜中，与FW相比，我们发现在I/II期严重程度时，TET1转录本和蛋白水平显著降低（分别为p = 0.001和p = 0.003），在III/IV期严重程度时仅转录本水平降低（p = 0.003）。在IWE的卵泡中期在位内膜中，与FW相比，在III/IV期严重程度时TET2转录本水平有统计学意义的降低（p = 0.037）。与卵泡中期内膜相比，我们发现所有IWE在位内膜黄体中期TET3转录本水平有统计学意义的升高（p = 0.034），在III/IV期严重程度时也是如此（p = 0.025）。我们观察到IWE在位内膜中TET1-3的表达水平发生了变化。
