Huang Yajia, Xia Aiguo, Yang Guang, Jin Fan
Department of Biomedical Engineering, College of Life Science and Technology , Huazhong University of Science and Technology , Wuhan 430074 , PR China.
Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, CAS Key Laboratory of Soft Matter Chemistry , University of Science and Technology of China , Hefei 230026 , PR China.
ACS Synth Biol. 2018 May 18;7(5):1195-1200. doi: 10.1021/acssynbio.8b00003. Epub 2018 Apr 18.
In this paper, we present a new strategy for microprinting dense bacterial communities with a prescribed organization on a substrate. Unlike conventional bioprinting techniques that require bioinks, through optogenetic manipulation, we directly manipulated the behaviors of Pseudomonas aeruginosa to allow these living bacteria to autonomically form patterned biofilms following prescribed illumination. The results showed that through optogenetic manipulation, patterned bacterial communities with high spatial resolution (approximately 10 μm) could be constructed in 6 h. Thus, optogenetic manipulation greatly increases the range of available bioprinting techniques.
在本文中，我们提出了一种在基底上微打印具有特定组织结构的密集细菌群落的新策略。与需要生物墨水的传统生物打印技术不同，通过光遗传学操作，我们直接操纵铜绿假单胞菌的行为，使这些活细菌在规定的光照下自主形成图案化生物膜。结果表明，通过光遗传学操作，可在6小时内构建出具有高空间分辨率（约10μm）的图案化细菌群落。因此，光遗传学操作极大地扩展了可用生物打印技术的范围。
