Institute of Environmental Engineering, Faculty of Infrastructure and Environment, Czestochowa University of Technology, Czestochowa, Poland.
Université Lille Nord de France, LGCgE-Lille 1, Ecologie Numérique et Ecotoxicologie, F-59650 Villeneuve d'Ascq, France.
J Environ Manage. 2018 Jul 15;218:71-78. doi: 10.1016/j.jenvman.2018.04.052. Epub 2018 Apr 14.
The aim of the study was to evaluate sensitivity and potential applications of selected biomarkers in phytoremediation under complex heavy metal contamination in Sinapis alba L., Robinia pseudoacacia L. and Lupinus luteus L as a potential tools in effective phytoremediation management. The toxicity assessment was conducted using selected measurement endpoints, both classical and advanced, i.e., germination index, roots length, guaiacol peroxidase activity (GPX), chlorophyll and protein content, the amount of total phenolic compounds (TPC) and level of expression of one of the ribulose-bisphosphate carboxylase genes (rbcL). Moreover, the influence of organic additives: cattle, horse manure, and vermicompost on lowering plant abiotic stress caused by complex heavy metal contamination was studied to assess the possible applications of selected stress markers in large scale phytoremediation planning. The results demonstrated the beneficial effects of selected soil additives on plant development. The 5% difference in the quantity of applied amendment caused statistically significant differences in GPX, TPC, chlorophyll content and expression level of rbcL. Among all endpoints, GPX activity, chlorophyll, and phenolic compounds content, as well as the expression of rbcL, turned out to be the most reliable assays for determination of the type and dosage of selected soil amendments (fertilizers) in the assisted phytoremediation process. Selected markers can be used to achieve the desired level of plant abiotic stress and consequently photosynthesis efficiency and CO sequestration. The results showed, that presented assays can be used in different taxonomical groups such as Fabaceae for planning effective phytoremediation process.
本研究旨在评估在复杂重金属污染条件下，芥菜（Sinapis alba L.）、刺槐（Robinia pseudoacacia L.）和羽扇豆（Lupinus luteus L.）中选定生物标志物的敏感性及其在植物修复中的潜在应用，作为有效植物修复管理的潜在工具。采用选定的测量终点，包括经典和先进的终点，即发芽指数、根长、愈创木酚过氧化物酶活性（GPX）、叶绿素和蛋白质含量、总酚含量（TPC）和核酮糖-1,5-二磷酸羧化酶基因（rbcL）的表达水平，对毒性进行评估。此外，还研究了有机添加剂（牛、马粪和蚯蚓粪）对降低复杂重金属污染引起的植物非生物胁迫的影响，以评估选定胁迫标志物在大规模植物修复规划中的可能应用。结果表明，选定的土壤添加剂对植物发育有有益的影响。应用的添加剂数量相差 5%，在 GPX、TPC、叶绿素含量和 rbcL 的表达水平上存在统计学差异。在所有终点中，GPX 活性、叶绿素和酚类化合物含量以及 rbcL 的表达被证明是确定辅助植物修复过程中选定土壤改良剂（肥料）类型和剂量的最可靠测定方法。选定的标志物可用于实现植物非生物胁迫和光合作用效率及 CO2 固存的预期水平。结果表明，所提出的测定方法可用于不同分类群，如豆科植物，以规划有效的植物修复过程。
