Jouvin M H, Rozenbaum W, Russo R, Kazatchkine M D
Unité d'Immunopathologie, Hôpital Broussais, Paris, France.
AIDS. 1987 Jul;1(2):89-94.
Expression of the C3b/C4b receptor (CR1) was studied on erythrocytes of 153 individuals infected with HIV and 104 age-matched normal individuals by measuring the uptake of 125I-labelled monoclonal anti-CR1 antibody. The mean number of CR1 sites on erythrocytes of asymptomatic seropositive individuals (822 +/- 270; mean +/- s.d.) and of patients with persistent generalized lymphadenopathy (PGL; 775 +/- 320) did not differ significantly from that of normal subjects. The number was significantly lower in patients with AIDS-related complex (ARC; 543 +/- 233; P less than 5 x 10(-3)) and further decreased in patients with AIDS (442 +/- 271; P less than 1 x 10(-4)), whether they presented with Kaposi's sarcoma (KS) or opportunistic infections. An additional finding was that of decreased expression of antigenic and functional CR1 in neutrophils from patients with AIDS, as assessed by radioimmunoassay of CR1 in detergent-solubilized cells and the capacity of intact cells to form rosettes with C3b-coated erythrocytes. Low numbers of CR1 on cells from patients with AIDS were not due to occupation of the receptor by C3 fragments on immune complexes. The correlation that was observed between decreased numbers of CR1 on erythrocytes and clinical subpopulations of symptomatic HIV-infected patients suggests that CR1 expression on erythrocytes may represent a valuable marker of the severity and natural history of HIV-associated disease.
通过测量125I标记的单克隆抗C3b/C4b受体（CR1）抗体的摄取量，研究了153例感染HIV的个体和104例年龄匹配的正常个体红细胞上CR1的表达情况。无症状血清阳性个体（822±270；均值±标准差）和持续性全身性淋巴结病（PGL）患者（775±320）红细胞上CR1位点的平均数与正常受试者相比无显著差异。艾滋病相关综合征（ARC）患者的该数值显著降低（543±233；P<5×10-3），而艾滋病患者（442±271；P<1×10-4）的数值进一步降低，无论他们是否患有卡波西肉瘤（KS）或机会性感染。另一个发现是，通过对去污剂溶解细胞中的CR1进行放射免疫测定以及完整细胞与C3b包被红细胞形成花环的能力评估，艾滋病患者中性粒细胞中抗原性和功能性CR1的表达降低。艾滋病患者细胞上CR1数量低并非由于免疫复合物上的C3片段占据了受体。在有症状的HIV感染患者的红细胞上CR1数量减少与临床亚群之间观察到的相关性表明，红细胞上CR1的表达可能是HIV相关疾病严重程度和自然史的一个有价值的标志物。
