Taheripourfard Zahra Sadat, Izadi-Darbandi Ali, Ghazvini Habibollah, Ebrahimi Mohsen, Mortazavian Seyed Mohammad Mahdi
Department of Agronomy and Plant Breeding Sciences, College of Aburaihan, University of Tehran, Tehran 3391653755, Iran.
J Genet. 2018 Mar;97(1):87-95.
Requirement of vernalization is an important factor which plays a crucial role in cereals to transit from vegetative to reproductive phase. There are three types of growth habit in barley: winter, spring and facultative types; in which spring type does not require vernalization but winter and facultative genotypes require full and partial vernalization, respectively. Combination of two loci, Vrn-h1 and Vrn-h2, regulates vernalization in barley genotypes. Specific DNA markers have been identified for growth habit regulator genes in barley. In this study, we examined 24 barley genotypes using specific primers for detecting Vrn-h1and Vrn-h2 loci. Results showed that among all differently suggested primer combinations, a few markers were precisely correlated with seasonal growth habit in barley. The specific markers of 600, 600 and 200 bps were verified for ZCCT-Ha, ZCCT-Hb and ZCCT-Hc loci, respectively. Our field growth habit test showed that cultivar Bahman as a winter growth habit, where all the others genotypes exhibited spring growth habit. By using specific primers for Vrn-h1, only Bahman cultivar produced 616 bp and 830 bp fragments and spring genotypes showed 574 bp or 616 bp alleles without any amplification for 830 bp fragments. Therefore, presence of 616 bp and 830 bp alleles together in each genotype can be considered as an informative marker for winter growth habit in barley. These informative markers can be used easily in barley breeding programmes for detection of growth habit types in the seedling stage.
春化需求是谷类作物从营养生长阶段过渡到生殖阶段的一个重要因素。大麦有三种生长习性类型：冬性、春性和兼性类型；其中春性类型不需要春化，而冬性和兼性基因型分别需要完全和部分春化。Vrn-h1和Vrn-h2这两个位点的组合调节大麦基因型的春化过程。已鉴定出大麦生长习性调控基因的特异性DNA标记。在本研究中，我们使用检测Vrn-h1和Vrn-h2位点的特异性引物对24个大麦基因型进行了检测。结果表明，在所有不同建议的引物组合中，有一些标记与大麦的季节性生长习性精确相关。分别验证了ZCCT-Ha、ZCCT-Hb和ZCCT-Hc位点的600、600和200 bp的特异性标记。我们的田间生长习性试验表明，品种Bahman为冬性生长习性，而其他所有基因型均表现为春性生长习性。通过使用Vrn-h1的特异性引物，只有Bahman品种产生了616 bp和830 bp的片段，而春性基因型显示574 bp或616 bp的等位基因，830 bp片段无扩增。因此，每个基因型中同时存在616 bp和830 bp的等位基因可被视为大麦冬性生长习性的一个信息性标记。这些信息性标记可轻松用于大麦育种计划，以在苗期检测生长习性类型。
