Knyazev E N, Mal'tseva D V, Zakharyants A A, Zakharova G S, Zhidkova O V, Poloznikov A A
BioClinicum Research Center, Moscow, Russia.
State Research Center Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
Bull Exp Biol Med. 2018 Apr;164(6):749-752. doi: 10.1007/s10517-018-4072-3. Epub 2018 Apr 17.
We studied expression profile of microRNA and their target genes in human umbilical vein endothelial cells (HUVEC) during proinflammatory activation with TNFα. TNFα-induced activation of HUVEC was accompanied by a decrease in the expression of CDKN1B, HIST1H3D, and OIP5 genes that are the common target genes for mature microRNA encoded by MIR221, MIR222, and MIR181B1 genes, whose expression increases in activated cells. Proteins encoded by HIST1H3D and OIP5 genes are associated with chromatin compaction and cell cycle. Our results suggest that fetal endothelial microRNA can appear in the maternal blood during various pathological states, e.g., under conditions of preeclampsia.
我们研究了在肿瘤坏死因子α（TNFα）促炎激活过程中，人脐静脉内皮细胞（HUVEC）中微小RNA及其靶基因的表达谱。TNFα诱导的HUVEC激活伴随着细胞周期蛋白依赖性激酶抑制剂1B（CDKN1B）、组蛋白H3D（HIST1H3D）和致癌相关蛋白5（OIP5）基因表达的降低，这些基因是由MIR221、MIR222和MIR181B1基因编码的成熟微小RNA的共同靶基因，其表达在激活的细胞中增加。HIST1H3D和OIP5基因编码的蛋白质与染色质压缩和细胞周期相关。我们的结果表明，胎儿内皮微小RNA可在各种病理状态下，如子痫前期的情况下出现在母体血液中。
