Zhang Lu, Wang Yan, Liu Dongxin, Luo Lijuan, Wang Yi, Ye Changyun
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing, China.
Front Microbiol. 2018 Apr 4;9:621. doi: 10.3389/fmicb.2018.00621. eCollection 2018.
, an important food-borne pathogen, causes listeriosis and is widely distributed in many different environments. In a previous study, we developed a novel enrichment broth containing D-allose that allows better isolation of from samples. However, the mechanism of D-allose utilization by remains unclear. In the present study, we determined the metabolism of D-allose in and found that lineage II strains of can utilize D-allose as the sole carbon source for growth, but lineage I and III strains cannot. Transcriptome analysis and sequence alignment identified six genes ( to ) possibly related to D-allose metabolism that are only present in the genomes of lineage II strains. Recombinant strain ICDC-LM188 containing these genes showed utilization of D-allose by growth assays and Biolog phenotype microarrays. Moreover, to were verified to be essential for D-allose metabolism, and affected the growth rate of in D-allose medium, while was dispensable in the metabolism of D-allose in . This is the first study to identify the genes related to D-allose metabolism in , and their distribution in lineage II strains. Our study preliminarily determined the effects of these genes on the growth of , which will benefit the isolation and epidemiological research of .
作为一种重要的食源性病原体，可引发李斯特菌病，广泛分布于许多不同环境中。在之前的一项研究中，我们开发了一种含有D - 阿洛糖的新型富集肉汤，能更好地从样本中分离出该菌。然而，该菌利用D - 阿洛糖的机制仍不清楚。在本研究中，我们确定了该菌中D - 阿洛糖的代谢情况，发现该菌的II系菌株能够利用D - 阿洛糖作为唯一碳源进行生长，但I系和III系菌株不能。转录组分析和序列比对鉴定出六个可能与D - 阿洛糖代谢相关的基因（至），这些基因仅存在于II系菌株的基因组中。含有这些基因的重组菌株ICDC - LM188通过生长试验和Biolog表型微阵列显示出对D - 阿洛糖的利用。此外，至被证实对D - 阿洛糖代谢至关重要，和影响该菌在D - 阿洛糖培养基中的生长速率，而在该菌的D - 阿洛糖代谢中可有可无。这是首次鉴定出与该菌中D - 阿洛糖代谢相关的基因及其在II系菌株中的分布的研究。我们的研究初步确定了这些基因对该菌生长的影响，这将有利于该菌的分离和流行病学研究。
