Baldión Paula A, Velandia-Romero Myriam L, Castellanos Jaime E
Grupo de Investigaciones Básicas y Aplicadas en Odontología, Universidad Nacional de Colombia, Bogotá, Colombia.
Grupo de Virología, Universidad El Bosque, Bogotá, Colombia.
Int J Cell Biol. 2018 Feb 19;2018:6853189. doi: 10.1155/2018/6853189. eCollection 2018.
Odontoblasts, the main cell type in teeth pulp tissue, are not cultivable and they are responsible for the first line of response after dental restauration. Studies on dental materials cytotoxicity and odontoblast cells physiology require large quantity of homogenous cells retaining most of the phenotype characteristics. Odontoblast-like cells (OLC) were differentiated from human dental pulp stem cells using differentiation medium (containing TGF-1), and OLC expanded after trypsinization (EXP-21) were evaluated and compared. Despite a slower cell growth curve, EXP-21 cells express similarly the odontoblast markers dentinal sialophosphoprotein and dentin matrix protein-1 concomitantly with RUNX2 transcripts and low alkaline phosphatase activity as expected. Both OLC and EXP-21 cells showed similar mineral deposition activity evidenced by alizarin red and von Kossa staining. These results pointed out minor changes in phenotype of subcultured EXP-21 regarding the primarily differentiated OLC, making the subcultivation of these cells a useful strategy to obtain odontoblasts for biocompatibility or cell physiology studies in dentistry.
成牙本质细胞是牙髓组织中的主要细胞类型，不可培养，且在牙齿修复后负责第一道反应。对牙科材料细胞毒性和成牙本质细胞生理学的研究需要大量保留大部分表型特征的同质细胞。使用分化培养基（含转化生长因子-1）从人牙髓干细胞中分化出成牙本质样细胞（OLC），并对胰蛋白酶消化后扩增的OLC（EXP-21）进行评估和比较。尽管细胞生长曲线较平缓，但EXP-21细胞如预期那样，与RUNX2转录本同时，类似地表达成牙本质细胞标志物牙本质涎磷蛋白和牙本质基质蛋白-1，且碱性磷酸酶活性较低。茜素红和冯科萨染色证明，OLC和EXP-21细胞均显示出相似的矿化沉积活性。这些结果表明，传代培养的EXP-21与最初分化的OLC相比，表型变化较小，使得这些细胞的传代培养成为一种获取成牙本质细胞用于牙科生物相容性或细胞生理学研究的有用策略。
