Muniandy Katyakyini, Gothai Sivapragasam, Tan Woan Sean, Kumar S Suresh, Mohd Esa Norhaizan, Chandramohan Govindasamy, Al-Numair Khalid S, Arulselvan Palanisamy
Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Evid Based Complement Alternat Med. 2018 Feb 19;2018:3142073. doi: 10.1155/2018/3142073. eCollection 2018.
Impaired wound healing is one of the serious problems among the diabetic patients. Currently, available treatments are limited due to side effects and cost effectiveness. In line with that, we attempted to use a natural source to study its potential towards the wound healing process. Therefore, (), an edible and medicinal plant, was chosen as the target sample for the study. During this investigation, the wound closure properties using stem extract of were analyzed. Accordingly, we analyzed the extract on free radical scavenging capacity and the cell migration of two most prominent cell types on the skin, human dermal fibroblast (NHDF), keratinocytes (HaCaT), and diabetic human dermal fibroblast (HDF-D) to mimic the wound healing in diabetic patients. The bioactive compounds were identified using gas chromatography-mass spectrometry (GC-MS). We discovered that the analysis exhibited a remarkable antioxidant, proliferative, and migratory rate in NHDF, HaCaT, and HDF-D in dose-dependent manner, which supports wound healing process, due to the presence of wound healing associated phytocompounds such as Hexadecanoic acid. This study suggested that the stem extract of might be a potential therapeutic agent for skin wound healing, supporting its traditional medicinal uses.
伤口愈合受损是糖尿病患者面临的严重问题之一。目前，由于副作用和成本效益，可用的治疗方法有限。与此一致的是，我们试图利用一种天然来源来研究其对伤口愈合过程的潜力。因此，选择了一种可食用且具有药用价值的植物作为该研究的目标样本。在这项研究中，分析了该植物茎提取物的伤口闭合特性。相应地，我们分析了该提取物对自由基清除能力以及皮肤中两种最主要细胞类型，即人皮肤成纤维细胞（NHDF）、角质形成细胞（HaCaT）和糖尿病患者人皮肤成纤维细胞（HDF-D）的细胞迁移的影响，以模拟糖尿病患者的伤口愈合情况。使用气相色谱 - 质谱联用（GC-MS）鉴定了生物活性化合物。我们发现，分析显示该提取物在NHDF、HaCaT和HDF-D中呈现出显著的抗氧化、增殖和迁移率，且呈剂量依赖性，这支持了伤口愈合过程，因为存在与伤口愈合相关的植物化合物，如十六烷酸。这项研究表明，该植物的茎提取物可能是一种用于皮肤伤口愈合的潜在治疗剂，这支持了其传统药用价值。
