Galal Maha Mohammed, Saad Ahmed Sayed
Analytical Chemistry Department, Faculty of Pharmacy, Cairo University Kasr El-Aini St, PO 11562 Cairo Egypt
Pharmaceutical Chemistry Department, School of Pharmacy and Pharmaceutical Industries, Badr University in Cairo (BUC) Badr City 11829 Cairo Egypt.
RSC Adv. 2020 Nov 24;10(70):42699-42705. doi: 10.1039/d0ra07070j. eCollection 2020 Nov 23.
During cancer treatment, doses must be carefully administered and monitored to guarantee efficacy and minimize side-effects. A potentiometric sensor was developed for the direct real-time assay of a widely used antineoplastic drug (vinblastine (VB)) in plasma samples. Membrane cocktails were drop-casted over a glassy-carbon electrode coated with a lipophilic conducting polymer (polyaniline). The study investigated five cation exchangers, five plasticizers (of different polarities and dielectric constants), and four ionophores with different physicochemical characters on the sensor performance. The study substantiates a data-driven selection of the optimum membrane recipe. The latter included sodium tetraphenylborate as an ion exchanger, dioctylphthalate as a plasticizer, and hydroxypropyl-β-cyclodextrin as ionophore. The membrane proved a near-Nernstian slope of 37.5 mV per decade, a LOQ of 2.99 × 10 M, and a stable fast response. The selectivity study proved poor responses to common physiological ions. The developed sensor was used for the determination of VB in its pure powder form, marketed formulation, and plasma samples. The fast and direct sensor response enables a wide range of applications in quality control laboratories and clinical studies.
在癌症治疗期间，必须谨慎给药并进行监测，以确保疗效并将副作用降至最低。开发了一种电位传感器，用于直接实时检测血浆样本中广泛使用的抗肿瘤药物（长春碱（VB））。将膜混合液滴铸在涂有亲脂性导电聚合物（聚苯胺）的玻碳电极上。该研究考察了五种阳离子交换剂、五种（具有不同极性和介电常数的）增塑剂以及四种具有不同物理化学特性的离子载体对传感器性能的影响。该研究证实了基于数据驱动的最佳膜配方选择。后者包括作为离子交换剂的四苯硼酸钠、作为增塑剂的邻苯二甲酸二辛酯以及作为离子载体的羟丙基-β-环糊精。该膜表现出每十倍浓度变化37.5 mV的近能斯特斜率、2.99×10 M的定量限以及稳定快速的响应。选择性研究表明，该传感器对常见生理离子的响应较差。所开发的传感器用于测定纯粉末形式、市售制剂以及血浆样本中的VB。这种快速直接的传感器响应使其能够在质量控制实验室和临床研究中得到广泛应用。
