Giordano M, Hagenmeyer-Houser S H, Sanberg P R
Department of Psychiatry, University of Cincinnati College of Medicine, OH 45267-0559.
Brain Res. 1988 Apr 12;446(1):183-8. doi: 10.1016/0006-8993(88)91313-3.
Striatal kainic acid (KA) lesions induce behavioral and biochemical deficits which resemble symptoms encountered in patients suffering from Huntington's disease. In rats with KA lesions, fetal striatal transplants have shown to reverse the pervasive nocturnal hyperactivity induced by the lesion. In the present study 4.6 mm3 of fetal striatal tissue were delivered bilaterally into the anterodorsal portion of the lesioned caudate nucleus. Care was taken to deliver the transplant within the host parenchyma and away from the lateral ventricles. Locomotor behavior analyzed using the Digiscan animal activity monitors before and after the transplants demonstrated a reversal of the hyperactivity following transplants in 70% of lesioned animals. Microinjections of horseradish peroxidase delivered into the globus pallidus and substantia nigra of a small group of functionally recovered transplanted animals, did not reveal evidence for reinnervation between host nigra or pallidum and the transplant at 10 weeks post-transplantation. Other laboratories have reported anatomical connections by 6 months post-transplantation. Ventricular/brain ratios demonstrated that intraparenchymal transplants significantly reduced the ventricular dilation following KA lesion. These results suggest that functional recovery can be obtained when the transplant is immersed into the host's striatal parenchyma regardless of the existence of long-range anatomical connections.
纹状体注射 kainic 酸（KA）会导致行为和生化缺陷，这些缺陷类似于亨廷顿舞蹈病患者出现的症状。在患有 KA 损伤的大鼠中，胎儿纹状体移植已显示可逆转由损伤引起的普遍夜间多动。在本研究中，将 4.6 立方毫米的胎儿纹状体组织双侧植入损伤尾状核的前背部分。小心地将移植物植入宿主实质内并远离侧脑室。在移植前后使用 Digiscan 动物活动监测器分析运动行为，结果表明 70%的损伤动物在移植后多动症状出现逆转。对一小部分功能恢复的移植动物的苍白球和黑质进行辣根过氧化物酶微注射，在移植后 10 周未发现宿主黑质或苍白球与移植物之间有重新支配的证据。其他实验室报告在移植后 6 个月有解剖学连接。脑室/脑比率表明，实质内移植显著减少了 KA 损伤后的脑室扩张。这些结果表明，当移植物植入宿主纹状体实质内时，无论是否存在长程解剖学连接，都可实现功能恢复。
