Hagenmeyer-Houser S H, Sanberg P R
Department of Psychology, Ohio University, Athens 45701.
Pharmacol Biochem Behav. 1987 Jul;27(3):583-6. doi: 10.1016/0091-3057(87)90372-8.
It is well established that embryonic tissue transplantation into an abnormal or lesioned brain can ameliorate some of the accompanying symptomatology. Specifically, transplants placed into kainic acid (KA) or ibotenic acid lesioned striatal rats promote behavioral recovery in various ambulatory measures. In the KA animal model, when the transplant encroached on normal host tissue, the behavioral recovery was diminished. However, little has been done to reveal what effect tissue transplants have on normal host brain. The present study placed E-17 striatal tissue into a normal adult striatum. Digiscan locomotor testing revealed that ten weeks after surgery, the implanted animals demonstrated pervasive nocturnal hyperactivity. Ambulatory, vertical and stereotypic measures were significantly increased when compared to controls. Rats with ten week implants showed lower increases in body gain yet increased food consumption when compared to controls. The transplants survived and contained normal looking AChE positively stained neurons. Evidence for fiber passage through the host-graft interface was also seen. When comparing three and ten week implants, there was a decrease in transplant size in the latter group accompanied by enlarged ventricles giving the brain a lesioned-like appearance. From these results, it is suggested that the placement of E-17 striatal tissue into adult striatum results in lesion-like behavior which may be attributed to the physical disruption of striatal systems.
胚胎组织移植到异常或受损大脑中能够改善一些伴随的症状，这一点已得到充分证实。具体而言，将移植组织植入经 kainic 酸（KA）或鹅膏蕈氨酸损伤的纹状体大鼠体内，可促进多种自主运动指标的行为恢复。在 KA 动物模型中，当移植组织侵入正常宿主组织时，行为恢复会减弱。然而，关于组织移植对正常宿主大脑有何影响的研究却很少。本研究将 E - 17 纹状体组织植入正常成年大鼠的纹状体。Digiscan 运动测试显示，术后十周，植入动物表现出普遍的夜间多动。与对照组相比，自主运动、垂直运动和刻板运动指标均显著增加。与对照组相比，植入十周的大鼠体重增加较少，但食物消耗量增加。移植组织存活下来，含有外观正常的乙酰胆碱酯酶（AChE）阳性染色神经元。还观察到有纤维穿过宿主 - 移植界面的证据。比较植入三周和十周的大鼠时，后一组的移植组织体积减小，脑室扩大，使大脑呈现出类似损伤的外观。从这些结果来看，将 E - 17 纹状体组织植入成年大鼠纹状体可导致类似损伤的行为，这可能归因于纹状体系统的物理破坏。
