牛中21-类固醇羟化酶、αA-晶体蛋白和I类牛白细胞抗原基因的同线映射。

Synteny mapping of the genes for 21 steroid hydroxylase, alpha A crystallin, and class I bovine leukocyte antigen in cattle.

作者信息

Skow L C, Womack J E, Petresh J M, Miller W L

机构信息

Department of Veterinary Anatomy, Texas A & M University, College Station 77843-4458.

出版信息

DNA. 1988 Apr;7(3):143-9. doi: 10.1089/dna.1988.7.143.

DOI:10.1089/dna.1988.7.143

PMID:2967168

Abstract

Synteny mapping of the genes for bovine alpha A crystallin (CRYA-1), steroid 21 hydroxylase (21OH), and a class I major histocompatibility locus bovine leukocyte antigen (BoLA) was accomplished by Southern blot analysis of DNA from bovine-hamster hybrid somatic cells. Comparison of the distribution patterns of CRYA-1, 21OH, and BoLA sequences among the hybrid cells revealed that the genes are located on two different chromosomes in the bovine genome. CRYA-1 mapped with superoxide dismutase (SOD1 in syntenic group U10, while 21OH and BoLA were concordant with glyoxylase (GLO1) in syntenic group U21. Therefore, the syntenic array of CRYA-1, 21OH, and BoLA in cattle is different from that observed in rodents, where the genes are closely linked to each other and to GLO1, and consistent with reported linkages in man.

摘要

通过对牛 - 仓鼠杂交体细胞的DNA进行Southern印迹分析，完成了牛αA晶状体蛋白（CRYA - 1）、类固醇21羟化酶（21OH）和I类主要组织相容性位点牛白细胞抗原（BoLA）基因的同线映射。对杂交细胞中CRYA - 1、21OH和BoLA序列分布模式的比较表明，这些基因位于牛基因组的两条不同染色体上。CRYA - 1与同线群U10中的超氧化物歧化酶（SOD1）同线定位，而21OH和BoLA与同线群U21中的乙二醛酶（GLO1）一致。因此，牛中CRYA - 1、21OH和BoLA的同线排列与在啮齿动物中观察到的不同，在啮齿动物中这些基因彼此紧密连锁并与GLO1连锁，且与人类中报道的连锁情况一致。