Polan M L, Daniele A, Kuo A
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06510-8063.
Fertil Steril. 1988 Jun;49(6):964-8.
Interleukin-1 (IL-1), a monocyte and macrophage product, is the mediator of a wide variety of immune responses, including fever, and increases during the luteal phase of the menstrual cycle, concomitantly with progesterone (P). These studies were undertaken to assess the possible relationship between steroidogenesis and IL-1 activity elaborated by human peripheral monocytes. IL-1 was measured with the D-10 thymocyte stimulation bioassay and dose response curves as a function of both estradiol (E2) and P were established. Low concentrations of both E2 (10(-9) M-10(-10) M) and P (10(-8) M-10(-9) M) resulted in maximal IL-1 stimulation. At higher concentrations of both E2 (10(-7) M) and P (10(-7) M-10(-5) M) there was a significant reduction (P less than 0.05) in IL-1 activity. However, neither E2 nor P production by human granulosa-luteal cells cultured with increasing amounts of human IL-1 (0 to 20 IU/ml) was affected in either the absence or presence of human chorionic gonadotropin (hCG, 5 IU/ml). Thus, human peripheral monocyte IL-1 activity appears to be modulated by gonadal steroids; however, a reciprocal relationship between IL-1 and gonadal steroidogenesis was not observed.
白细胞介素-1（IL-1）是单核细胞和巨噬细胞的产物，是多种免疫反应的介质，包括发热，在月经周期的黄体期会升高，与孕酮（P）同时出现。进行这些研究是为了评估类固醇生成与人类外周血单核细胞产生的IL-1活性之间的可能关系。用D-10胸腺细胞刺激生物测定法测量IL-1，并建立了作为雌二醇（E2）和P函数的剂量反应曲线。低浓度的E2（10^-9 M - 10^-10 M）和P（10^-8 M - 10^-9 M）均可导致IL-1的最大刺激。在较高浓度的E2（10^-7 M）和P（10^-7 M - 10^-5 M）时，IL-1活性显著降低（P < 0.05）。然而，在添加或不添加人绒毛膜促性腺激素（hCG，5 IU/ml）的情况下，用增加量的人IL-1（0至20 IU/ml）培养的人颗粒黄体细胞产生的E2和P均未受到影响。因此，人类外周血单核细胞的IL-1活性似乎受性腺类固醇调节；然而，未观察到IL-1与性腺类固醇生成之间的相互关系。
