Department of Rehabilitation Medicine of Korean Medicine, Mokpo Korean Hospital of Dongshin University, 313 Baengnyeon-daero, Mokpo-si, 58665, Republic of Korea.
College of Korean Medicine, Dongshin University, 185 Geonjae-ro, Naju-si, Jeollanam-do, 58245, Republic of Korea.
BMC Complement Altern Med. 2018 Apr 19;18(1):131. doi: 10.1186/s12906-018-2149-1.
Previously, we reported that ChondorT showed significant anti-arthritis and anti-inflammatory effects. ChondroT, a new herbal medication, consists of the water extracts of Osterici Radix, Lonicerae Folium, Angelicae Gigantis Radix, Clematidis Radix, and Phellodendri Cortex (6:4:4:4:3). The objective of this study was to investigate the effects of ChondroT in collagenase-induced osteoarthritis rat model.
Osteoarthritis was induced by the injection of collagenase into the right knee joint cavity of rats. The samples were divided into seven groups [intact (n = 6), control (n = 6), indomethacin (n = 6), Joins tab (n = 6), ChondroT50 (n = 6), ChondroT100 (n = 6), and ChondroT200 (n = 6)]. The control group was administered normal saline, indomethacin group was administered indomethacin (2 mg/kg), and Joins tab group was administered Joins Tab (20 mg/kg). The ChondroT50, ChondroT100, and ChondroT200 groups were administered 50, 100, and 200 mg/kg of ChondroT, respectively. All oral administrations were initiated 7 days after the induction of arthritis and were continued for a total of 12 days. At the end of the experiment, serum aminotransferase, albumin, blood urea nitrogen, creatinine, leukocyte, and inflammatory cytokines [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] were analyzed. Hematoxylin and eosin (H&E) and safranin O-fast green staining of the articular structures of the knee joint were performed.
TNF-α and IL-1β decreased in the ChondroT100 and ChondroT200 groups compared with those in the control group. IL-6 and aspartate aminotransferase decreased in the ChondroT50, ChondroT100, and ChondroT200 groups compared with that in the control group. Albumin, WBC and lymphocytes decreased in the ChondroT100 and ChondroT200 groups compared with those in the control group. In H&E stain, synoviocytes, cartilage lacunae, and chondrocytes were well preserved in the ChondroT100 and ChondroT200 groups, and safranin O-fast staining showed a clear reaction of proteoglycans in the ChondroT100 and ChondroT200 groups.
Based on these results, it can be proposed that ChondroT has anti-osteoarthritic effects on collagenase-induced rat model.
我们之前报道过，ChondorT 具有显著的抗关节炎和抗炎作用。ChondroT 是一种新的草药药物，由独活根、金银花叶、当归根、威灵仙根和黄柏皮的水提取物组成（6:4:4:4:3）。本研究旨在探讨 ChondroT 对胶原酶诱导的骨关节炎大鼠模型的影响。
通过向大鼠右膝关节腔注射胶原酶诱导骨关节炎。将样本分为七组[完整组（n = 6）、对照组（n = 6）、吲哚美辛组（n = 6）、Joins 片组（n = 6）、ChondroT50 组（n = 6）、ChondroT100 组（n = 6）和 ChondroT200 组（n = 6）]。对照组给予生理盐水，吲哚美辛组给予吲哚美辛（2mg/kg），Joins 片组给予 Joins 片（20mg/kg）。ChondroT50、ChondroT100 和 ChondroT200 组分别给予 50、100 和 200mg/kg 的 ChondroT。所有口服给药均在关节炎诱导后 7 天开始，并持续 12 天。实验结束时，分析血清转氨酶、白蛋白、血尿素氮、肌酐、白细胞和炎症细胞因子[肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和 IL-6]。对膝关节关节结构进行苏木精和伊红（H&E）和番红 O-快绿染色。
与对照组相比，ChondroT100 和 ChondroT200 组的 TNF-α 和 IL-1β 降低。与对照组相比，ChondroT50、ChondroT100 和 ChondroT200 组的 IL-6 和天门冬氨酸氨基转移酶降低。与对照组相比，ChondroT100 和 ChondroT200 组的白蛋白、白细胞和淋巴细胞减少。在 H&E 染色中，ChondroT100 和 ChondroT200 组的滑膜细胞、软骨陷窝和软骨细胞保存良好，番红 O-快绿染色显示 ChondroT100 和 ChondroT200 组的蛋白聚糖有明显反应。
基于这些结果，可以提出 ChondroT 对胶原酶诱导的大鼠模型具有抗骨关节炎作用。
